ArticleFEMS yeast research2013
Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications.
Article in FEMS yeast research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 226 citations in OpenAlex.
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- A hybrid system enables plasmid copy number control in yeast.Nature communications · 2026Article
- Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases.Microbial cell factories · 2026Article
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- How many plasmids can bacteria carry? A synthetic biology perspective.Open biology · 2025Review
- Reduced Genetic Heterogeneity for Stable Bioproduction by Harnessing the Bias and Mechanism of Mutation.Microbial biotechnology · 2025Article
- A predatory gene drive for targeted control of self-transmissible plasmids.Science advances · 2025Article
- Metabolic engineering of Escherichia coli for enhanced production of p-coumaric acid via L-phenylalanine biosynthesis pathway.Bioprocess and biosystems engineering · 2025Article
- Pulcherriminic acid biosynthesis and transport: insights from a heterologous system in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Engineering Inorganic Pyrophosphate Metabolism as a Strategy to Generate a Fluoride-ResistantMicroorganisms · 2025Article
- A versatile genetic toolkit for engineeringFrontiers in bioengineering and biotechnology · 2025Article
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- Omics-driven onboarding of the carotenoid producing red yeast Xanthophyllomyces dendrorhous CBS 6938.Applied microbiology and biotechnology · 2024Article
- A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells.FEBS open bio · 2024Article
- The middle domain of Hsp104 can ensure substrates are functional after processing.PLoS genetics · 2024Article
- Evolving dual-trait EPSP synthase variants using a synthetic yeast selection system.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Strategic targeting of Cas9 nickase induces large segmental duplications.Cell genomics · 2024Article
- Plasticity of the mitotic spindle in response to karyotype variation.Current biology : CB · 2024Article
46 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
Abstract
Many metabolic engineering and genetic engineering applications in yeast rely on the use of plasmids. Despite their pervasive use and the diverse collections available, there is a fundamental lack of understanding of how commonly used DNA plasmids affect the cell's ability to grow and how the choice of plasmid components can influence plasmid load and burden. In this study, we characterized the major attributes of the 2 micron and centromeric plasmids typically used in yeast by examining the impact of choice of selection marker, promoter, origin of replication, and strain ploidy on conferred growth rates and plasmid copy number. We conclude that the 'plasmid burden', as demonstrated by a reduced growth rate, is primarily due to the choice of selection marker, especially when auxotrophic markers are utilized. The plasmid burden traditionally attributed to replication and maintenance of plasmid DNA plays only a minor role in haploid yeast yet is much more significant in diploid strains. The selection marker can also significantly change plasmid copy number. In fact, plasmid copy number can be influenced to some extent by all of the parameters tested. The information presented in this study will allow for more rational design and selection of plasmids for engineering applications.
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