ArticleNeurogastroenterology and motility2012
Full-field optical coherence microscopy is a novel technique for imaging enteric ganglia in the gastrointestinal tract.
Article in Neurogastroenterology and motility, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- High-resolution dynamic full-field optical coherence microscopy: illuminating intracellular activity in deep tissue.Npj imaging · 2026Article
- Current and future state of the management of Hirschsprung disease.World journal of pediatric surgery · 2025Review
- Visualizing enteric nervous system activity through dye-free dynamic full-field optical coherence tomography.Communications biology · 2023Article
- Assessment of Gastrointestinal Autonomic Dysfunction: Present and Future Perspectives.Journal of clinical medicine · 2021Review
- Fingerprint imaging from the inside of a finger with full-field optical coherence tomography.Biomedical optics express · 2015Article
- Imaging: a novel technique for imaging enteric ganglia.Nature reviews. Gastroenterology & hepatology · 2012Article
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11 authors.
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Abstract
backgroundNoninvasive methods are needed to improve the diagnosis of enteric neuropathies. Full-field optical coherence microscopy (FFOCM) is a novel optical microscopy modality that can acquire 1 μm resolution images of tissue. The objective of this research was to demonstrate FFOCM imaging for the characterization of the enteric nervous system (ENS).
methodsNormal mice and EdnrB(-/-) mice, a model of Hirschsprung's disease (HD), were imaged in three-dimensions ex vivo using FFOCM through the entire thickness and length of the gut. Quantitative analysis of myenteric ganglia was performed on FFOCM images obtained from whole-mount tissues and compared with immunohistochemistry imaged by confocal microscopy. KEY
resultsFull-field optical coherence microscopy enabled visualization of the full thickness gut wall from serosa to mucosa. Images of the myenteric plexus were successfully acquired from the stomach, duodenum, colon, and rectum. Quantification of ganglionic neuronal counts on FFOCM images revealed strong interobserver agreement and identical values to those obtained by immunofluorescence microscopy. In EdnrB(-/-) mice, FFOCM analysis revealed a significant decrease in ganglia density along the colorectum and a significantly lower density of ganglia in all colorectal segments compared with normal mice. CONCLUSIONS & INFERENCES: Full-field optical coherence microscopy enables optical microscopic imaging of the ENS within the bowel wall along the entire intestine. FFOCM is able to differentiate ganglionic from aganglionic colon in a mouse model of HD, and can provide quantitative assessment of ganglionic density. With further refinements that enable bowel wall imaging in vivo, this technology has the potential to revolutionize the characterization of the ENS and the diagnosis of enteric neuropathies.
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