ArticleJournal of neurochemistry2013
X-ray fluorescence analysis of iron and manganese distribution in primary dopaminergic neurons.
Article in Journal of neurochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Targeting chelatable iron as a therapeutic modality in Parkinson's disease.Antioxidants & redox signaling · 2014Trial
- Synchrotron XRF Imaging Reveals Manganese Accumulation in the Golgi and Post-Synapses of Neurons and Enhanced Uptake in Astrocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Integrating Microscopy Methods to Study Gene and Protein Expression alongside Metal Ion Distribution and Speciation: A Case Study of Iron within Pyramidal Neurons from Distinct Hippocampal CA1 Subregions.Chemical & biomedical imaging · 2026Article
- Manganese Neurotoxicity: A Comprehensive Review of Pathophysiology and Inherited and Acquired Disorders.Journal of xenobiotics · 2025Review
- Gut to brain: essential micronutrient and trace element manganese transport, function and toxicity.Frontiers in physiology · 2025Review
- Metal ion fluxes controlling amphibian fertilization.Nature chemistry · 2021Article
- Association Between Dietary Iron Intake and Serum Ferritin and Severe Headache or Migraine.Frontiers in nutrition · 2021Article
- Manganese Accumulation in the Brain via Various Transporters and Its Neurotoxicity Mechanisms.Molecules (Basel, Switzerland) · 2020Review
- Manganese-induced Mitochondrial Dysfunction Is Not Detectable at Exposures Below the Acute Cytotoxic Threshold in Neuronal Cell Types.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Nano-imaging trace elements at organelle levels in substantia nigra overexpressing α-synuclein to model Parkinson's disease.Communications biology · 2020Article
- New Insights on the Role of Manganese in Alzheimer's Disease and Parkinson's Disease.International journal of environmental research and public health · 2019Review
- SLC30A10 Mutation Involved in Parkinsonism Results in Manganese Accumulation within Nanovesicles of the Golgi Apparatus.ACS chemical neuroscience · 2019Article
- X-ray fluorescence imaging of metals and metalloids in biological systems.American journal of nuclear medicine and molecular imaging · 2018Review
- Redox dynamics of manganese as a mitochondrial life-death switch.Biochemical and biophysical research communications · 2017Review
- Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling.Analytical chemistry · 2017Review
- Bioavailable Trace Metals in Neurological Diseases.Current treatment options in neurology · 2016Review
- α-Synuclein Over-Expression Induces Increased Iron Accumulation and Redistribution in Iron-Exposed Neurons.Molecular neurobiology · 2016Article
- Visualizing Metal Content and Intracellular Distribution in Primary Hippocampal Neurons with Synchrotron X-Ray Fluorescence.PloS one · 2016Article
- Identification of dopaminergic neurons of the substantia nigra pars compacta as a target of manganese accumulation.Metallomics : integrated biometal science · 2015Article
- Preparing adherent cells for X-ray fluorescence imaging by chemical fixation.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transition metals have been suggested to play a pivotal role in the pathogenesis of Parkinson's disease. X-ray microscopy combined with a cryogenic setup is a powerful method for elemental imaging in low concentrations and high resolution in intact cells, eliminating the need for fixation and sectioning of the specimen. Here, we performed an elemental distribution analysis in cultured primary midbrain neurons with a step size in the order of 300 nm and ~ 0.1 ppm sensitivity under cryo conditions by using X-ray fluorescence microscopy. We report the elemental mappings on the subcellular level in primary mouse dopaminergic (DAergic) and non-DAergic neurons after treatment with transition metals. Application of Fe(2+) resulted in largely extracellular accumulation of iron without preference for the neuronal transmitter subtype. A quantification of different Fe oxidation states was performed using X-ray absorption near edge structure analysis. After treatment with Mn(2+) , a cytoplasmic/paranuclear localization of Mn was observed preferentially in DAergic neurons, while no prominent signal was detectable after Mn(3+) treatment. Immunocytochemical analysis correlated the preferential Mn uptake to increased expression of voltage-gated calcium channels in DAergic neurons. We discuss the implications of this differential elemental distribution for the selective vulnerability of DAergic neurons and Parkinson's disease pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.