ArticleSensors and actuators. B, Chemical2012
Fabrication of X-ray compatible microfluidic platforms for protein crystallization.
Article in Sensors and actuators. B, Chemical, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
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Who cites it
29 citing papers in PubMed.
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Cyclic Olefin Copolymer-Based Fixed-Target Sample Delivery Device for Protein X-ray Crystallography.Analytical chemistry · 2024Article
- Manufacturing of Ultra-Thin X-ray-Compatible COC Microfluidic Devices for Optimal In Situ Macromolecular Crystallography Experiments.Micromachines · 2022Article
- 3D-printed SAXS chamber for controlled in situ dialysis and optical characterization.Journal of synchrotron radiation · 2022Article
- Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation.Scientific reports · 2021Article
- Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.Lab on a chip · 2021Article
- Fabrication and characterisation of a silicon-borosilicate glass microfluidic device for synchrotron-based hard X-ray spectroscopy studies.RSC advances · 2021Article
- A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.Journal of applied crystallography · 2021Article
- Microfluidic characterization of macromolecular liquid-liquid phase separation.Lab on a chip · 2020Article
- Real-Time Measurement of Protein Crystal Growth Rates within the Microfluidic Device to Understand the Microspace Effect.ACS omega · 2020Article
- The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review.Micromachines · 2020Review
- X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds.Lab on a chip · 2018Article
- Crystallization via tubing microfluidics permits both in situ and ex situ X-ray diffraction.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Watching Proteins Function with Time-resolved X-ray Crystallography.Journal of physics D: Applied physics · 2017Article
- X-ray transparent microfluidic chips for high-throughput screening and optimization ofBiomicrofluidics · 2017Article
- Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography.Acta crystallographica. Section D, Structural biology · 2016Article
- A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions.Acta crystallographica. Section D, Biological crystallography · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
This paper reports a method for fabricating multilayer microfluidic protein crystallization platforms using different materials to achieve X-ray transparency and compatibility with crystallization reagents. To validate this approach, three soluble proteins, lysozyme, thaumatin, and ribonuclease A were crystallized on-chip, followed by on-chip diffraction data collection. We also report a chip with an array of wells for screening different conditions that consume a minimal amount of protein solution as compared to traditional screening methods. A large number of high quality isomorphous protein crystals can be grown in the wells, after which slices of X-ray data can be collected from many crystals still residing within the wells. Complete protein structures can be obtained by merging these slices of data followed by further processing with crystallography software. This approach of using an x-ray transparent chip for screening, crystal growth, and X-ray data collection enables room temperature data collection from many crystals mounted in parallel, which thus eliminates crystal handling and minimizes radiation damage to the crystals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.