ArticleJournal of experimental botany2012
Modulation of plant TPC channels by polyunsaturated fatty acids.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Review
- The Two-Pore Channel 2 in Human Physiology and Diseases: Functional Characterisation and Pharmacology.International journal of molecular sciences · 2025Review
- Electrophysiology and fluorescence to investigate cation channels and transporters in isolated plant vacuoles.Stress biology · 2022Review
- Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters.Cells · 2022Review
- Molecular Approaches Reduce Saturates and EliminateFrontiers in plant science · 2022Review
- Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.International journal of molecular sciences · 2021Article
- Review
- Structural basis for activation of voltage sensor domains in an ion channel TPC1.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Hidden Effects of Seed Quality Breeding on Germination in Oilseed Rape (Frontiers in plant science · 2018Article
- On the structure and mechanism of two-pore channels.The FEBS journal · 2018Article
- The human two-pore channel 1 is modulated by cytosolic and luminal calcium.Scientific reports · 2017Article
- Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels.Frontiers in physiology · 2017Review
- ROS, Calcium, and Electric Signals: Key Mediators of Rapid Systemic Signaling in Plants.Plant physiology · 2016Article
- The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix.Cellular and molecular life sciences : CMLS · 2016Article
- Two-pore Channels Enter the Atomic Era: Structure of Plant TPC Revealed.Trends in biochemical sciences · 2016Article
- Effect of Trimethyltin Chloride on Slow Vacuolar (SV) Channels in Vacuoles from Red Beet (Beta vulgaris L.) Taproots.PloS one · 2015Article
- The phosphoinositide PI(3,5)P₂ mediates activation of mammalian but not plant TPC proteins: functional expression of endolysosomal channels in yeast and plant cells.Cellular and molecular life sciences : CMLS · 2014Article
- Plant salt stress status is transmitted systemically via propagating calcium waves.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Plant calcium-permeable channels.Plant physiology · 2013Review
- Cation-permeable vacuolar ion channels in the moss Physcomitrella patens: a patch-clamp study.Planta · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana mesophyll cells and arachidonic acid (AA) was chosen as a model molecule for PUFAs. Our study was extended to different PUFAs including the endogenous alpha-linolenic acid (ALA). The addition of micromolar concentrations of AA reversibly inhibited the SV channel decreasing the maximum open probability and shifting the half activation voltage to positive values. Comparing the effects of different PUFAs, it was found that the length of the lipophilic acyl chain, the number of double bonds and the polar head were critical for channel modulation.The experimental data can be reproduced by a simple three-state model, in which PUFAs do not interact directly with the voltage sensors but affect the voltage-independent transition that leads the channel from the open state to the closed configuration. The results indicate that lipids play an important role in co-ordinating ion channel activities similar to what is known from animal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.