ArticleGenetics2013
Depleting gene activities in early Drosophila embryos with the "maternal-Gal4-shRNA" system.
Article in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- AbioRxiv : the preprint server for biology · 2026Article
- Resolving early embryonic metabolism in Drosophila through single-embryo metabolomics and transcriptomics.Nature metabolism · 2025Article
- Addendum: Retinoic acid signaling is critical during the totipotency window in early mammalian development.Nature structural & molecular biology · 2025Article
- Chemotaxis of Drosophila border cells is modulated by tissue geometry through dispersion of chemoattractants.iScience · 2025Article
- Multi-tissue characterization of the constitutive heterochromatin proteome in Drosophila identifies a link between satellite DNA organization and transposon repression.PLoS biology · 2025Article
- Integrated stress response signaling acts as a metabolic sensor in fat tissues to regulate oocyte maturation and ovulation.Cell reports · 2024Article
- Single-cell transcriptomics illuminates regulatory steps driving anterior-posterior patterning of Drosophila embryonic mesoderm.Cell reports · 2023Article
- Review
- Stepwise modifications of transcriptional hubs link pioneer factor activity to a burst of transcription.Nature communications · 2023Article
- CTCF, BEAF-32, and CP190 are not required for the establishment of TADs in earlyScience advances · 2023Article
- Rap1 regulates apical contractility to allow embryonic morphogenesis without tissue disruption and acts in part via Canoe-independent mechanisms.Molecular biology of the cell · 2023Article
- Astral microtubule cross-linking safeguards uniform nuclear distribution in the Drosophila syncytium.The Journal of cell biology · 2022Article
- Article
- TheFaculty reviews · 2022Review
- Spatial organization of transcribing loci during early genome activation in Drosophila.Current biology : CB · 2021Article
- Article
- Membrane architecture and adherens junctions contribute to strong Notch pathway activation.Development (Cambridge, England) · 2021Article
- Maternal Piwi regulates primordial germ cell development to ensure the fertility of female progeny in Drosophila.Genetics · 2021Article
- Gatekeeper function for Short stop at the ring canals of the Drosophila ovary.Current biology : CB · 2021Article
- Pak1 and PP2A antagonize aPKC function to support cortical tension induced by the Crumbs-Yurt complex.eLife · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
In a developing Drosophila melanogaster embryo, mRNAs have a maternal origin, a zygotic origin, or both. During the maternal-zygotic transition, maternal products are degraded and gene expression comes under the control of the zygotic genome. To interrogate the function of mRNAs that are both maternally and zygotically expressed, it is common to examine the embryonic phenotypes derived from female germline mosaics. Recently, the development of RNAi vectors based on short hairpin RNAs (shRNAs) effective during oogenesis has provided an alternative to producing germline clones. Here, we evaluate the efficacies of: (1) maternally loaded shRNAs to knockdown zygotic transcripts and (2) maternally loaded Gal4 protein to drive zygotic shRNA expression. We show that, while Gal4-driven shRNAs in the female germline very effectively generate phenotypes for genes expressed maternally, maternally loaded shRNAs are not very effective at generating phenotypes for early zygotic genes. However, maternally loaded Gal4 protein is very efficient at generating phenotypes for zygotic genes expressed during mid-embryogenesis. We apply this powerful and simple method to unravel the embryonic functions of a number of pleiotropic genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.