ArticleJournal of bacteriology2013
Characterization of a mazEF toxin-antitoxin homologue from Staphylococcus equorum.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Inhibition of Targeted Bacterial Growth Using MazF-Expressing Phagemids: A Novel Therapeutic Approach Based on Endoribonuclease Toxin Molecules.Microbiology and immunology · 2026Article
- MazEF Homologs inToxins · 2024Article
- Article
- Conserved Amino Acid Moieties ofFrontiers in microbiology · 2021Article
- Functional genomics reveals the toxin-antitoxin repertoire and AbiE activity inMicrobial genomics · 2020Article
- MazF Endoribonucleolytic Toxin Conserved inToxins · 2020Article
- The Variety in the Common Theme of Translation Inhibition by Type II Toxin-Antitoxin Systems.Frontiers in genetics · 2020Review
- Article
- Comparative genomic analysis of Staphylococcus lugdunensis shows a closed pan-genome and multiple barriers to horizontal gene transfer.BMC genomics · 2018Article
- Article
- Artificial Activation ofFrontiers in microbiology · 2018Article
- Identification of novel mazEF/pemIK family toxin-antitoxin loci and their distribution in the Staphylococcus genus.Scientific reports · 2017Article
- Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease.MicrobiologyOpen · 2017Article
- Toxins of Prokaryotic Toxin-Antitoxin Systems with Sequence-Specific Endoribonuclease Activity.Toxins · 2017Review
- The Influence of the Toxin/Antitoxin mazEF on Growth and Survival of Listeria monocytogenes under Stress.Toxins · 2017Article
- Cloaked dagger: tRNA slicing by an unlikely culprit.RNA biology · 2017Review
- Toxin-Antitoxin Systems of Staphylococcus aureus.Toxins · 2016Review
- Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing.PloS one · 2016Article
- The MazEF Toxin-Antitoxin System Alters the β-Lactam Susceptibility of Staphylococcus aureus.PloS one · 2015Article
- Fluorescence based primer extension technique to determine transcriptional starting points and cleavage sites of RNases in vivo.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Toxin-antitoxin (TA) systems encoded in prokaryotic genomes fall into five types, typically composed of two distinct small molecules, an endotoxic protein and a cis-encoded antitoxin of ribonucleic or proteinaceous nature. In silico analysis revealed seven putative type I and three putative type II TA systems in the genome of the nonpathogenic species strain Staphylococcus equorum SE3. Among these, a MazEF system orthologue termed MazEF(seq) was further characterized. 5' rapid amplification of cDNA ends (RACE) revealed the expression and the transcriptional start site of mazE(seq), indicating an immediately upstream promoter. Heterologous expression of the putative toxin-encoding mazF(seq) gene imposed growth cessation but not cell death on Escherichia coli. In vivo and in vitro, MazF(seq) was shown to cleave at UACAU motifs, which are remarkably abundant in a number of putative metabolic and regulatory S. equorum gene transcripts. Specific interaction between MazF(seq) and the putative cognate antitoxin MazE(seq) was demonstrated by bacterial two-hybrid analyses. These data strongly suggest that MazEF(seq) represents the first characterized TA system in a nonpathogenic Staphylococcus species and indicate that MazEF modules in staphylococci may also control processes beyond pathogenicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.