ArticleApplied and environmental microbiology2013
Fluorometric quantification of polyphosphate in environmental plankton samples: extraction protocols, matrix effects, and nucleic acid interference.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 93 citations in OpenAlex.
- The inositol pyrophosphate 5-InsPThe Journal of biological chemistry · 2026Article
- Phosphorus Acquisition Strategies Among Phytoplankton and Free-Living Bacterial Communities in the Baltic Proper.Environmental microbiology reports · 2026Article
- Review
- The microbial phosphorus cycle in aquatic ecosystems.Nature reviews. Microbiology · 2025Review
- Article
- Quantification of Polyphosphate in Environmental Planktonic Samples Using a Novel Fluorescence Dye JC-D7.Environmental science & technology · 2024Article
- Toolkit for cellular studies of mammalian mitochondrial inorganic polyphosphate.Frontiers in cell and developmental biology · 2023Article
- Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems.Frontiers in microbiology · 2023Article
- Comparison of photosynthetic responses between haptophyteFrontiers in microbiology · 2023Article
- Phosphate limitation intensifies negative effects of ocean acidification on globally important nitrogen fixing cyanobacterium.Nature communications · 2022Article
- Schindler's legacy: from eutrophic lakes to the phosphorus utilization strategies of cyanobacteria.FEMS microbiology reviews · 2022Review
- Dissolved organic phosphorus utilization by the marine bacterium Ruegeria pomeroyi DSS-3 reveals chain length-dependent polyphosphate degradation.Environmental microbiology · 2022Article
- Acclimation ofFrontiers in microbiology · 2022Article
- Fluorometric Quantification of Human Platelet Polyphosphate Using 4',6-Diamidine-2-phenylindole Dihydrochloride: Applications in the Japanese Population.International journal of molecular sciences · 2021Article
- Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility.International journal of implant dentistry · 2020Article
- Bacterial Phosphate Granules Contain Cyclic Polyphosphates: Evidence fromJournal of the American Chemical Society · 2020Article
- Fixing the Broken Phosphorus Cycle: Wastewater Remediation by Microalgal Polyphosphates.Frontiers in plant science · 2020Review
- Polyphosphate: A Multifunctional Metabolite in Cyanobacteria and Algae.Frontiers in plant science · 2020Review
- Characteristic Microbiomes Correlate with Polyphosphate Accumulation of Marine Sponges in South China Sea Areas.Microorganisms · 2019Article
- Picoplankton accumulate and recycle polyphosphate to support high primary productivity in coastal Lake Ontario.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyphosphate (polyP) is a ubiquitous biochemical with many cellular functions and comprises an important environmental phosphorus pool. However, methodological challenges have hampered routine quantification of polyP in environmental samples. We tested 15 protocols to extract inorganic polyphosphate from natural marine samples and cultured cyanobacteria for fluorometric quantification with 4',6-diamidino-2-phenylindole (DAPI) without prior purification. A combination of brief boiling and digestion with proteinase K was superior to all other protocols, including other enzymatic digestions and neutral or alkaline leaches. However, three successive extractions were required to extract all polyP. Standard addition revealed matrix effects that differed between sample types, causing polyP to be over- or underestimated by up to 50% in the samples tested here. Although previous studies judged that the presence of DNA would not complicate fluorometric quantification of polyP with DAPI, we show that RNA can cause significant interference at the wavelengths used to measure polyP. Importantly, treating samples with DNase and RNase before proteinase K digestion reduced fluorescence by up to 57%. We measured particulate polyP along a North Pacific coastal-to-open ocean transect and show that particulate polyP concentrations increased toward the open ocean. While our final method is optimized for marine particulate matter, different environmental sample types may need to be assessed for matrix effects, extraction efficiency, and nucleic acid interference.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.