ArticleNucleic acids research2013
Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 72 citations in OpenAlex.
- Enzyme-Responsive Cyclic Gapmer Antisense Oligonucleotides via ThymidineBioconjugate chemistry · 2026Article
- Topological Switch-OFF and β-Galactosidase-Triggered Switch-ON of Cyclic Antisense Oligonucleotides via CuAAC for Controlled RNA Cleavage.Bioconjugate chemistry · 2025Article
- RNA Control via Redox-Responsive Acylation.Angewandte Chemie (International ed. in English) · 2024Article
- Spatiotemporal control of RNA metabolism and CRISPR-Cas functions using engineered photoswitchable RNA-binding proteins.Nature protocols · 2024Review
- Antisense Oligonucleotide Activation via Enzymatic Antibiotic Resistance Mechanism.ACS chemical biology · 2023Article
- Cathepsin B-activatable cyclic antisense oligonucleotides for cell-specific target gene knockdownMolecular therapy. Nucleic acids · 2023Article
- Precise, Orthogonal Remote-Control of Cell-Free Systems Using Photocaged Nucleic Acids.Journal of the American Chemical Society · 2023Article
- Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins.Nature biotechnology · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Photoactivatable Circular Caged Oligonucleotides for TranscriptomeChemPhotoChem · 2021Article
- Article
- Designing Photolabile Ruthenium Polypyridyl Crosslinkers for Hydrogel Formation and Multiplexed, Visible-light Degradation.RSC advances · 2019Article
- Efficient Synthesis of Light-Triggered Circular Antisense Oligonucleotides Targeting Cellular Protein Expression.Chembiochem : a European journal of chemical biology · 2018Article
- RNA Control by Photoreversible Acylation.Journal of the American Chemical Society · 2018Article
- RNA Cloaking by Reversible Acylation.Angewandte Chemie (International ed. in English) · 2018Article
- Light-Activated Control of Translation by Enzymatic Covalent mRNA Labeling.Angewandte Chemie (International ed. in English) · 2018Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Circular siRNAs for Reducing Off-Target Effects and Enhancing Long-Term Gene Silencing in Cells and Mice.Molecular therapy. Nucleic acids · 2018Article
- Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides.Chemical science · 2018Article
- Caged circular siRNAs for photomodulation of gene expression in cells and mice.Chemical science · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We synthesized three 20 mer caged circular antisense oligodeoxynucleotides (R20, R20B2 and R20B4) with a photocleavable linker and an amide bond linker between two 10 mer oligodeoxynucleotides. With these caged circular antisense oligodeoxynucleotides, RNA-binding affinity and its digestion by ribonuclease H were readily photomodulated. RNA cleavage rates were upregulated ~43-, 25- and 15-fold for R20, R20B2 and R20B4, respectively, upon light activation in vitro. R20B2 and R20B4 with 2- or 4-nt gaps in the target RNA lost their ability to bind the target RNA even though a small amount of RNA digestion was still observed. The loss of binding ability indicated promising gene photoregulation through a non-enzymatic strategy. To test this strategy, three caged circular antisense oligonucleotides (PS1, PS2 and PS3) with 2'-OMe RNA and phosphorothioate modifications were synthesized to target GFP expression. Upon light activation, photomodulation of target hybridization and GFP expression in cells was successfully achieved with PS1, PS2 and PS3. These caged circular antisense oligonucleotides show promising applications of photomodulating gene expression through both ribonuclease H and non-enzyme involved antisense strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.