ArticlePlant signaling & behavior2013
Identification of an EMS-induced causal mutation in a gene required for boron-mediated root development by low-coverage genome re-sequencing in Arabidopsis.
Article in Plant signaling & behavior, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Genome-Wide Profile of Mutations Induced by Carbon Ion Beam Irradiation of Dehulled Rice Seeds.International journal of molecular sciences · 2024Article
- What Can Boron Deficiency Symptoms Tell Us about Its Function and Regulation?Plants (Basel, Switzerland) · 2023Review
- Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review.Plants (Basel, Switzerland) · 2020Review
- An SMU Splicing Factor Complex Within Nuclear Speckles Contributes to Magnesium Homeostasis in Arabidopsis.Plant physiology · 2020Article
- Combining next-generation sequencing and progeny testing for rapid identification of induced recessive and dominant mutations in maize MThe Plant journal : for cell and molecular biology · 2019Article
- Genome-Wide Comparisons of Mutations Induced by Carbon-Ion Beam and Gamma-Rays Irradiation in RiceFrontiers in plant science · 2019Article
- Article
- Screening Arabidopsis thaliana mutants for low sensitivity to manganese identifies novel alleles of NRAMP1 and PGSIP6.Journal of experimental botany · 2018Article
- Identification and characterization of suppressor mutants of stop1.BMC plant biology · 2017Article
- Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation inFrontiers in plant science · 2017Article
- Polyamine Resistance Is Increased by Mutations in a Nitrate Transporter Gene NRT1.3 (AtNPF6.4) in Arabidopsis thaliana.Frontiers in plant science · 2016Article
- An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production.Journal of experimental botany · 2015Article
- Localization of causal locus in the genome of the brown macroalga Ectocarpus: NGS-based mapping and positional cloning approaches.Frontiers in plant science · 2015Article
- Article
- Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis.EMBO reports · 2014Article
- Maturation processes and structures of small secreted peptides in plants.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Next-generation sequencing (NGS) technologies enable the rapid production of an enormous quantity of sequence data. These powerful new technologies allow the identification of mutations by whole-genome sequencing. However, most reported NGS-based mapping methods, which are based on bulked segregant analysis, are costly and laborious. To address these limitations, we designed a versatile NGS-based mapping method that consists of a combination of low- to medium-coverage multiplex SOLiD (Sequencing by Oligonucleotide Ligation and Detection) and classical genetic rough mapping. Using only low to medium coverage reduces the SOLiD sequencing costs and, since just 10 to 20 mutant F 2 plants are required for rough mapping, the operation is simple enough to handle in a laboratory with limited space and funding. As a proof of principle, we successfully applied this method to identify the CTR1, which is involved in boron-mediated root development, from among a population of high boron requiring Arabidopsis thaliana mutants. Our work demonstrates that this NGS-based mapping method is a moderately priced and versatile method that can readily be applied to other model organisms.
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