ArticleMolecular biology and evolution2013
Nonlinear fitness consequences of variation in expression level of a eukaryotic gene.
Article in Molecular biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
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- The shape of fitness functions and the distribution of mutational effect sizes jointly limit adaptation by regulatory mutations.Nature ecology & evolution · 2026Article
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- Molecular evolution in light of regulatory-coding epistasis.EMBO reports · 2026Review
- Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi.Cell systems · 2025Article
- The potential of inversions to accumulate balanced sexual antagonism is supported by simulations andeLife · 2025Article
- Plasticity and environment-specific relationships between gene expression and fitness in Saccharomyces cerevisiae.Nature ecology & evolution · 2024Article
- Plasticity and environment-specific relationships between gene expression and fitness inbioRxiv : the preprint server for biology · 2024Article
- Article
- Mechanisms of regulatory evolution in yeast.Current opinion in genetics & development · 2022Review
- On the incongruence of genotype-phenotype and fitness landscapes.PLoS computational biology · 2022Article
- Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow.Molecular ecology · 2022Article
- Article
- Molecular and evolutionary processes generating variation in gene expression.Nature reviews. Genetics · 2021Review
- Mismatch-CRISPRi Reveals the Co-varying Expression-Fitness Relationships of Essential Genes in Escherichia coli and Bacillus subtilis.Cell systems · 2020Article
- Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.Nature biotechnology · 2020Article
- DNA variants affecting the expression of numerous genes in trans have diverse mechanisms of action and evolutionary histories.PLoS genetics · 2019Article
- Empirical mean-noise fitness landscapes reveal the fitness impact of gene expression noise.Nature communications · 2019Article
- Dominance reversals and the maintenance of genetic variation for fitness.PLoS biology · 2019Article
- Heterosis Is a Systemic Property Emerging From Non-linear Genotype-Phenotype Relationships: Evidence FromFrontiers in genetics · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Levels of gene expression show considerable variation in eukaryotes, but no fine-scale maps have been made of the fitness consequences of such variation in controlled genetic backgrounds and environments. To address this, we assayed fitness at many levels of up- and down-regulated expression of a single essential gene, LCB2, involved in sphingolipid synthesis in budding yeast Saccharomyces cerevisiae. Reduced LCB2 expression rapidly decreases cellular fitness, yet increased expression has little effect. The wild-type expression level is therefore perched on the edge of a nonlinear fitness cliff. LCB2 is upregulated when cells are exposed to osmotic stress; consistent with this, the entire fitness curve is shifted upward to higher expression under osmotic stress, illustrating the selective force behind gene regulation. Expression levels of LCB2 are lower in wild yeast strains than in the experimental lab strain, suggesting that higher levels in the lab strain may be idiosyncratic. Reports indicate that the effect sizes of alleles contributing to variation in complex phenotypes differ among environments and genetic backgrounds; our results suggest that such differences may be explained as simple shifts in the position of nonlinear fitness curves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.