ArticleNature cell biology2012
β2-syntrophin and Par-3 promote an apicobasal Rac activity gradient at cell-cell junctions by differentially regulating Tiam1 activity.
Article in Nature cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 52 citations in OpenAlex.
- Autoregulation of the LIM kinases by their PDZ domain.Nature communications · 2023Article
- A TIAM1-TRIM28 complex mediates epigenetic silencing of protocadherins to promote migration of lung cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rho and Rab Family Small GTPases in the Regulation of Membrane Polarity in Epithelial Cells.Frontiers in cell and developmental biology · 2022Review
- The RAC1 activator Tiam1 regulates centriole duplication through controlling PLK4 levels.Journal of cell science · 2021Article
- Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane.American journal of physiology. Cell physiology · 2020Article
- The interaction between CASK and the tumour suppressor Dlg1 regulates mitotic spindle orientation in mammalian epithelia.Journal of cell science · 2019Article
- [Polarity of ameloblasts and odontoblasts and their related regulators].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2019Review
- TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer.Cell death & disease · 2019Article
- Spatial Rho regulation: Molecular mechanisms controlling the GAP protein DLC3.Small GTPases · 2019Article
- Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.International review of cell and molecular biology · 2019Review
- Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.Nature cell biology · 2018Article
- Signaling by Small GTPases at Cell-Cell Junctions: Protein Interactions Building Control and Networks.Cold Spring Harbor perspectives in biology · 2018Review
- Altered expression of TIAM1 in endotoxin-challenged airway epithelial cells and rodent septic models.Journal of thoracic disease · 2018Article
- Making Heads or Tails of It: Cell-Cell Adhesion in Cellular and Supracellular Polarity in Collective Migration.Cold Spring Harbor perspectives in biology · 2017Review
- TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis.Cell death and differentiation · 2017Article
- The scaffold protein Ajuba suppresses CdGAP activity in epithelia to maintain stable cell-cell contacts.Scientific reports · 2017Article
- An apicobasal gradient of Rac activity determines protrusion form and position.Nature communications · 2017Article
- TIAM1 Antagonizes TAZ/YAP Both in the Destruction Complex in the Cytoplasm and in the Nucleus to Inhibit Invasion of Intestinal Epithelial Cells.Cancer cell · 2017Article
- Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity.Molecular biology of the cell · 2017Article
- Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis.Molecular medicine reports · 2016Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 3 countries.
Funding
Abstract
Although Rac and its activator Tiam1 are known to stimulate cell-cell adhesion, the mechanisms regulating their activity in cell-cell junction formation are poorly understood. Here, we identify β2-syntrophin as a Tiam1 interactor required for optimal cell-cell adhesion. We show that during tight-junction (TJ) assembly β2-syntrophin promotes Tiam1-Rac activity, in contrast to the function of the apical determinant Par-3 whose inhibition of Tiam1-Rac activity is necessary for TJ assembly. We further demonstrate that β2-syntrophin localizes more basally than Par-3 at cell-cell junctions, thus generating an apicobasal Rac activity gradient at developing cell-cell junctions. Targeting active Rac to TJs shows that this gradient is required for optimal TJ assembly and apical lumen formation. Consistently, β2-syntrophin depletion perturbs Tiam1 and Rac localization at cell-cell junctions and causes defects in apical lumen formation. We conclude that β2-syntrophin and Par-3 fine-tune Rac activity along cell-cell junctions controlling TJ assembly and the establishment of apicobasal polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.