Evidence map›Paper›PMID 23103470›Full record

ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2012

Large-scale development of cost-effective single-nucleotide polymorphism marker assays for genetic mapping in pigeonpea and comparative mapping in legumes.

Rachit K Saxena, R Varma Penmetsa, Hari D Upadhyaya, Ashish Kumar, Noelia Carrasquilla-Garcia, Jessica A Schlueter, Andrew Farmer, Adam M Whaley, Birinchi K Sarma, Gregory D May and 2 more

Open access · goldAbstract readComparative Study
In one paragraph

Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
10.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 112 citations in OpenAlex.

  1. Breeding for next-generation biotic stress-tolerant pigeonpea for sustainable food legume production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Review
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  11. Breeding potential of lablab [Genetic resources and crop evolution · 2021
    Review
  12. Article
  13. A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021
    Article
  14. Article
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  18. Genomics-assisted breeding for pigeonpea improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 3 countries.

Rachit K SaxenaCenter of Excellence in Genomics (CEG), International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Patancheru 502324, India.
R Varma Penmetsa
Hari D Upadhyaya
Ashish Kumar
Noelia Carrasquilla-Garcia
Jessica A Schlueter
Andrew Farmer
Adam M Whaley
Birinchi K Sarma
Gregory D May
Douglas R Cook
Rajeev K Varshney
University of California, Davis · USInternational Crops Research Institute for the Semi-Arid Tropics · INNational Center for Genome Resources · USUniversity of North Carolina at Charlotte · USDepartment of Plant Biology · USJawaharlal Nehru Krishi Vishwa Vidyalaya · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-nucleotide polymorphisms (SNPs, >2000) were discovered by using RNA-seq and allele-specific sequencing approaches in pigeonpea (Cajanus cajan). For making the SNP genotyping cost-effective, successful competitive allele-specific polymerase chain reaction (KASPar) assays were developed for 1616 SNPs and referred to as PKAMs (pigeonpea KASPar assay markers). Screening of PKAMs on 24 genotypes [23 from cultivated species and 1 wild species (Cajanus scarabaeoides)] defined a set of 1154 polymorphic markers (77.4%) with a polymorphism information content (PIC) value from 0.04 to 0.38. One thousand and ninety-four PKAMs showed polymorphisms between parental lines of the reference mapping population (C. cajan ICP 28 × C. scarabaeoides ICPW 94). By using high-quality marker genotyping data on 167 F(2) lines from the population, a comprehensive genetic map comprising 875 PKAMs with an average inter-marker distance of 1.11 cM was developed. Previously mapped 35 simple sequence repeat markers were integrated into the PKAM map and an integrated genetic map of 996.21 cM was constructed. Mapped PKAMs showed a higher degree of synteny with the genome of Glycine max followed by Medicago truncatula and Lotus japonicus and least with Vigna unguiculata. These PKAMs will be useful for genetics research and breeding applications in pigeonpea and for utilizing genome information from other legume species.

Indexed as

AllelesBase SequenceCajanusChromosome MappingCost-Benefit AnalysisFabaceaeGene FrequencyGenetic LinkageGenetic MarkersGenomicsGenotypeHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsMolecular Sequence DataPolymorphism, Single NucleotideSequence Analysis, DNAGenetic Markers

Identifiers

PMID23103470
PMCPMC3514856
OpenAlexW2101149707

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.