ArticleMolecular cell2012
Distinct single-cell morphological dynamics under beta-lactam antibiotics.
Article in Molecular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
119 citing papers in PubMed, 2 syntheses or guidelines pooled it, 206 citations in OpenAlex.
- Pharmacokinetic-pharmacodynamic modeling using combined time-kill data for meropenem and colistin or polymyxin B combination effects inAntimicrobial agents and chemotherapy · 2026Pooled it
- Tackling the Antibiotic Resistance Caused by Class AInternational journal of molecular sciences · 2018Pooled it
- The Direct and Potentiating Antimicrobial Activities of Zafirlukast.Antibiotics (Basel, Switzerland) · 2026Article
- Bacterial growth under confinement requires transcriptional adaptation to resist turgor pressure build-up.Nature communications · 2026Article
- A microfluidic hollow-fiber infection model (µHFIM): monitoring bacterial response to dynamic drug treatment with single-cell resolution.Microsystems & nanoengineering · 2026Article
- The Brief Case: How antibiotic pretreatment complicated the diagnosis ofJournal of clinical microbiology · 2025Review
- Mechanics ofMolecular biology of the cell · 2025Article
- Peptidoglycan-outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria.Nature microbiology · 2025Article
- Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli.BMC microbiology · 2025Article
- Saccharin disrupts bacterial cell envelope stability and interferes with DNA replication dynamics.EMBO molecular medicine · 2025Article
- β-Barrel proteins dictate the effect of core oligosaccharide composition on outer membrane mechanics.Biophysical journal · 2025Article
- Resensitizing β-Lactams by Reprogramming Purine Metabolism in Small Colony Variant for Osteomyelitis Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Antibiotic tolerance among clinical isolates: mechanisms, detection, prevalence, and significance.Clinical microbiology reviews · 2024Review
- Anisotropic patterns of nanospikes induces anti-biofouling and mechano-bactericidal effects of titanium nanosurfaces with electrical cue.Materials today. Bio · 2024Article
- Exploiting β-Lactams-Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual-Digital PCR.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- β-barrel proteins dictate the effect of core oligosaccharide composition on outer membrane mechanics.bioRxiv : the preprint server for biology · 2024Article
- Article
- Energy allocation theory for bacterial growth control in and out of steady state.bioRxiv : the preprint server for biology · 2024Article
- Growth Independent Morphometric Machine Learning Workflow for Single-Cell Antimicrobial Susceptibility Testing ofFrontiers in imaging (Lausanne, Switzerland) · 2024Article
- Chemical genetic approaches for the discovery of bacterial cell wall inhibitors.RSC medicinal chemistry · 2023Review
59 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The bacterial cell wall is conserved in prokaryotes, stabilizing cells against osmotic stress. Beta-lactams inhibit cell-wall synthesis and induce lysis through a bulge-mediated mechanism; however, little is known about the formation dynamics and stability of these bulges. To capture processes of different timescales, we developed an imaging platform combining automated image analysis with live-cell microscopy at high time resolution. Beta-lactam killing of Escherichia coli cells proceeded through four stages: elongation, bulge formation, bulge stagnation, and lysis. Both the cell wall and outer membrane (OM) affect the observed dynamics; damaging the cell wall with different beta-lactams and compromising OM integrity cause different modes and rates of lysis. Our results show that the bulge-formation dynamics are determined by how the cell wall is perturbed. The OM plays an independent role in stabilizing the bulge once it is formed. The stabilized bulge delays lysis and allows recovery upon drug removal.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.