ArticleBiomaterials2013
Proteomic analysis of a decellularized human vocal fold mucosa scaffold using 2D electrophoresis and high-resolution mass spectrometry.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations.Exploration (Beijing, China) · 2025Review
- Engineering a 3D platform for testis bioengineering: generation and proteomic profiling of decellularized fish testicular scaffolds.Frontiers in bioengineering and biotechnology · 2025Article
- Unraveling the Relevance of Tissue-Specific Decellularized Extracellular Matrix Hydrogels for Vocal Fold Regenerative Biomaterials: A Comprehensive Proteomic and In Vitro Study.Advanced nanobiomed research · 2023Article
- Functional acellular matrix for tissue repair.Materials today. Bio · 2023Review
- Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation.Laryngoscope investigative otolaryngology · 2022Article
- Furosemide-induced systemic dehydration alters the proteome of rabbit vocal folds.Journal of proteomics · 2022Article
- Ovary-derived Decellularized Extracellular Matrix-based Bioink for Fabricating 3D Primary Ovarian Cells-laden Structures for Mouse Ovarian Failure Correction.International journal of bioprinting · 2022Article
- Bioactive potential of natural biomaterials: identification, retention and assessment of biological properties.Signal transduction and targeted therapy · 2021Review
- Acellular Biologic Nipple-Areolar Complex Graft:Tissue engineering. Part A · 2020Article
- Bioengineered airway epithelial grafts with mucociliary function based on collagen IV- and laminin-containing extracellular matrix scaffolds.The European respiratory journal · 2020Article
- A tissue-specific, injectable acellular gel for the treatment of chronic vocal fold scarring.Acta biomaterialia · 2019Article
- In Depth Quantification of Extracellular Matrix Proteins from Human Pancreas.Journal of proteome research · 2019Article
- Practice variations in voice treatment selection following vocal fold mucosal resection.The Laryngoscope · 2016Article
- Extracellular matrix-based biomaterial scaffolds and the host response.Biomaterials · 2016Review
- Protein turnover during in vitro tissue engineering.Biomaterials · 2016Article
- Elucidating the Molecular Composition of Cartilage by Proteomics.Journal of proteome research · 2016Article
- Quantification of decellularized human myocardial matrix: A comparison of six patients.Proteomics. Clinical applications · 2016Article
- Proteomic analysis of naturally-sourced biological scaffolds.Biomaterials · 2016Article
- Bioengineered vocal fold mucosa for voice restoration.Science translational medicine · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Natural biologic scaffolds for tissue engineering are commonly generated by decellularization of tissues and organs. Despite some preclinical and clinical success, in vivo scaffold remodeling and functional outcomes remain variable, presumably due to the influence of unidentified bioactive molecules on the scaffold-host interaction. Here, we used 2D electrophoresis and high-resolution mass spectrometry-based proteomic analyses to evaluate decellularization effectiveness and identify potentially bioactive protein remnants in a human vocal fold mucosa model. We noted proteome, phosphoproteome and O-glycoproteome depletion post-decellularization, and identified >200 unique protein species within the decellularized scaffold. Gene ontology-based enrichment analysis revealed a dominant set of functionally-related ontology terms associated with extracellular matrix assembly, organization, morphology and patterning, consistent with preservation of a tissue-specific niche for later cell seeding and infiltration. We further identified a subset of ontology terms associated with bioactive (some of which are antigenic) cellular proteins, despite histological and immunohistochemical data indicating complete decellularization. These findings demonstrate the value of mass spectrometry-based proteomics in identifying agents potentially responsible for variation in host response to engineered tissues derived from decellularized scaffolds. This work has implications for the manufacturing of biologic scaffolds from any tissue or organ, as well as for prediction and monitoring of the scaffold-host interaction in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.