ReviewTrends in biotechnology2012
Engineering Escherichia coli to synthesize free fatty acids.
Review in Trends in biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 194 citations in OpenAlex.
- Organelle-Targeted Multi-Enzyme Engineering Enables Medium-Chain Fatty Acid Production inJournal of microbiology and biotechnology · 2026Article
- Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli.Metabolic engineering · 2026Article
- A metabolic engineering strategy for producing poly-(3-hydroxyoctanoic acid) in Escherichia coli from glycerol.Metabolic engineering · 2025Article
- CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies inSynthetic and systems biotechnology · 2025Article
- In silico identification of gene targets to enhance C12 fatty acid production in Escherichia coli.Applied microbiology and biotechnology · 2025Article
- A Fast-Pass, Desorption Electrospray Ionization Mass Spectrometry Strategy for Untargeted Metabolic Phenotyping.Journal of the American Society for Mass Spectrometry · 2025Article
- Click chemistry-enabled gold nanorods for sensitive detection and viability evaluation of copper(II)-reducing bacteria.Materials today. Bio · 2025Article
- Triple-Gene Overexpression of the AcrA-AcrB-TolC Transporter System inInternational journal of molecular sciences · 2024Article
- "Metabolic burden" explained: stress symptoms and its related responses induced by (over)expression of (heterologous) proteins in Escherichia coli.Microbial cell factories · 2024Review
- Pseudomonas putida as a platform for medium-chain length α,ω-diol production: Opportunities and challenges.Microbial biotechnology · 2024Article
- Machine learning model of the catalytic efficiency and substrate specificity of acyl-ACP thioesterase variants generated from natural andFrontiers in bioengineering and biotechnology · 2024Article
- Tuning Fatty Acid Profile and Yield inBioengineering (Basel, Switzerland) · 2023Article
- Longitudinal Single-Cell Imaging of Engineered Strains with Stimulated Raman Scattering to Characterize Heterogeneity in Fatty Acid Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Determinants of substrate specificity in a catalytically diverse family of acyl-ACP thioesterases from plants.BMC plant biology · 2023Article
- Microbial pathways for advanced biofuel production.Biochemical Society transactions · 2022Review
- Overview of the Cellular Stress Responses Involved in Fatty Acid Overproduction in E. coli.Molecular biotechnology · 2022Review
- Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity.Nature communications · 2022Article
- Thioesterase enzyme families: Functions, structures, and mechanisms.Protein science : a publication of the Protein Society · 2022Article
- Extracellular Electron Transfer Enables Cellular Control of Cu(I)-Catalyzed Alkyne-Azide Cycloaddition.ACS central science · 2022Article
- Accelerating strain phenotyping with desorption electrospray ionization-imaging mass spectrometry and untargeted analysis of intact microbial colonies.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Fatty acid metabolism has received significant attention as a route for producing high-energy density, liquid transportation fuels and high-value oleochemicals from renewable feedstocks. If microbes can be engineered to produce these compounds at yields that approach the theoretical limits of 0.3-0.4 g/g glucose, then processes can be developed to replace current petrochemical technologies. Here, we review recent metabolic engineering efforts to maximize production of free fatty acids (FFA) in Escherichia coli, the first step towards production of downstream products. To date, metabolic engineers have succeeded in achieving higher yields of FFA than any downstream products. Regulation of fatty acid metabolism and the physiological effects of fatty acid production will also be reviewed from the perspective of identifying future engineering targets.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.