ArticleIndian journal of microbiology2009
Strain improvement of Trichoderma reesei Rut C-30 for increased cellulase production.
Article in Indian journal of microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 34 citations in OpenAlex.
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
- Improvement of the capacity to hydrolyze corncob residues through deciphering the lignocellulolytic enzyme system of a hyper-cellulolytic mutant strain Trichoderma reesei CU7-4.Biotechnology for biofuels and bioproducts · 2025Article
- Genome Sequencing and Comparative Genomics of the Hyper-Cellulolytic FungusJournal of fungi (Basel, Switzerland) · 2025Article
- The Role of Cell Wall Degrading Enzymes in Antagonistic Traits ofIranian journal of biotechnology · 2020Article
- The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases inInternational journal of molecular sciences · 2019Article
- Is an organic nitrogen source needed for cellulase production by Trichoderma reesei Rut-C30?World journal of microbiology & biotechnology · 2013Article
- Development of a β-glucosidase hyperproducing mutant by combined chemical and UV mutagenesis.3 Biotech · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The strain of Trichoderma reesei Rut C-30 was subjected to mutation after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NG) for 6 h followed by UV irradiation for 15 min. Successive mutants showed enhanced cellulase production, clear hydrolysis zone and rapid growth on Avicel-containing plate. Particularly, the mutant NU-6 showed approximately two-fold increases in activity of both FPA and CMCase in shake flask culture when grown on basal medium containing peptone (1%) and wheat bran (1%). The enzyme production was further optimized using eight different media. When a mixture of lactose and yeast cream was used as cellulase inducer, the mutant NU-6 yielded the highest enzyme and cell production with a FPase activity of 6.2 U ml(-1), a CMCase activity of 54.2 U ml(-1), a β-glucosidase activity of 0.39 U ml(-1), and a fungal biomass of 12.6 mg ml(-1). It deserved noting that the mutant NU-6 also secreted large amounts of xylanases (291.3 U ml(-1)). These results suggested that NU-6 should be an attractive producer for both cellulose and xylanase production.
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