ArticleIndian journal of microbiology2008
Production of β-galactosidase by Kluyveromyces marxianus MTCC 1388 using whey and effect of four different methods of enzyme extraction on β-galactosidase activity.
Article in Indian journal of microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- Probiotic Yeasts and Bacteria: A Complementary Approach to Gut Health.Microorganisms · 2026Review
- Physiological characterization ofFrontiers in microbiology · 2025Article
- Concerning the presumptive identification of Candida kefyr on Uriselect™4 agar.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2024Article
- A New Extracellular β-Galactosidase ProducingIndian journal of microbiology · 2021Article
- The identification of novel promoters and terminators for protein expression and metabolic engineering applications inMetabolic engineering communications · 2021Article
- Exploitation of Ultrasound Technique for Enhancement of Microbial Metabolites Production.Molecules (Basel, Switzerland) · 2020Review
- Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model.BMC bioinformatics · 2019Article
- Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of β-cryptoxanthin byRoyal Society open science · 2018Article
- β-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design.Iranian biomedical journal · 2016Article
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4 authors at 1 institution in 1 country.
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Abstract
Whey containing 4.4% (w/v) lactose was inoculated with Kluyveromyces marxianus MTCC 1389 for carrying out studies related to β-galactosidase production. β-galactosidase activity was found to be maximum after 30 h and further incubation resulted in decline in activity. The maximum cell biomass of 2.54 mg mL(-1) was observed after 36 h of incubation. Lactose concentration dropped drastically to 0.04 % from 4.40% after 36 h of incubation. Out of the four methods tested for extraction of enzyme, SDS - Chlorofom method was found to be best followed by Toluene - Acetone, sonication and homogenization with glass beads in that order. It could be concluded through this study that SDS - Chloroform is cheap and simple method for enzyme extraction from Kluyveromyces cells, which resulted in higher enzyme activity as compared to the activity observed using the remaining extraction methods. The study could also establish that whey could effectively be utilized for β-galactosidase production thus alleviating water pollution problems caused due to its disposal into the water streams.
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