ArticleThe Journal of infectious diseases2013
Radiation-induced cellular and molecular alterations in asexual intraerythrocytic Plasmodium falciparum.
Article in The Journal of infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Low-Intensity Light-Responsive Anticancer Activity of Platinum(II) Complex Nanocolloids on 2D and 3D In Vitro Cancer Cell Model.Bioinorganic chemistry and applications · 2022Article
- Article
- Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification.Malaria journal · 2019Article
- Transcriptome analysis based detection of Plasmodium falciparum development in Anopheles stephensi mosquitoes.Scientific reports · 2018Article
- Checks and balances? DNA replication and the cell cycle in Plasmodium.Parasites & vectors · 2018Review
- Induction of immunity following vaccination with a chemically attenuated malaria vaccine correlates with persistent antigenic stimulation.Clinical & translational immunology · 2018Article
- Molecular Markers of Radiation Induced Attenuation in Intrahepatic Plasmodium falciparum Parasites.PloS one · 2016Article
- Expression, Purification, and Biological Characterization of Babesia microti Apical Membrane Antigen 1.Infection and immunity · 2015Article
- DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum.Microbiology and molecular biology reviews : MMBR · 2014Review
- Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing.Biology direct · 2013Article
- The Cytotoxic Effects of Low Intensity Visible and Infrared Light on Human Breast Cancer (MCF7) cells.Computational and structural biotechnology journal · 2013Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundγ-irradiation is commonly used to create attenuation in Plasmodium parasites. However, there are no systematic studies on the survival, reversion of virulence, and molecular basis for γ-radiation-induced cell death in malaria parasites.
methodsThe effect of γ-irradiation on the growth of asexual Plasmodium falciparum was studied in erythrocyte cultures. Cellular and ultrastructural changes within the parasite were studied by fluorescence and electron microscopy, and genome-wide transcriptional profiling was performed to identify parasite biomarkers of attenuation and cell death.
resultsγ-radiation induced the death of P. falciparum in a dose-dependent manner. These parasites had defective mitosis, sparse cytoplasm, fewer ribosomes, disorganized and clumped organelles, and large vacuoles-observations consistent with "distressed" or dying parasites. A total of 185 parasite genes were transcriptionally altered in response to γ-irradiation (45.9% upregulated, 54.1% downregulated). Loss of parasite survival was correlated with the downregulation of genes encoding translation factors and with upregulation of genes associated with messenger RNA-sequestering stress granules. Genes pertaining to cell-surface interactions, host-cell remodeling, and secreted proteins were also altered.
conclusionsThese studies provide a framework to assess the safety of γ-irradiation attenuation and promising targets for genetic deletion to produce whole parasite-based attenuated vaccines.
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