ArticleNature protocols2012
Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
95 citing papers in PubMed, 227 citations in OpenAlex.
- YAP-mediated mechanotransduction promotes hepatocellular carcinoma progression by targeting ATP1A1/CXCL1 axis.Acta pharmacologica Sinica · 2026Article
- Role of semaphorin 4f in cardiac fibroblasts to regulate matrix production through actin remodeling and YAP/TAZ activation.Apoptosis : an international journal on programmed cell death · 2026Article
- Three-dimensional spheroid models in breast cancer: tumor microenvironment complexity, cancer stem cell-driven resistance, and translational model integration.Journal of translational medicine · 2026Review
- Fibronectin-induced overactivation of αNature communications · 2026Article
- Mechanobiology of the tumor microenvironment: a review of therapeutic interactions and in vitro elasticity measurement techniques.Journal of biomedical science · 2026Review
- Radical-Mediated, Substrate-Independent Fabrication of Hybrid Solid-Hydrogel Materials With Tunable Crosslinking: An Initiator- and Crosslinker-Free Approach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Dynamic insights into cellular mechanics and membrane undulations in vascular smooth muscle cells.American journal of physiology. Cell physiology · 2026Article
- Rethinking Matrigel: The Complex Journey to Matrix Alternatives in Organoid Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Curved Magnetic Hydrogels for Understanding Cancer Initiation.ACS applied materials & interfaces · 2025Article
- The functional role of the extracellular matrix in inflammatory bowel disease associated gut fibrosis.Matrix biology : journal of the International Society for Matrix Biology · 2025Review
- The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells.Discover oncology · 2025Article
- Article
- Hydrogels for Cardiac Tissue Regeneration: Current and Future Developments.International journal of molecular sciences · 2025Review
- Article
- Semiaromatic Polyester-Ethers with Tunable Degradation Profiles.ACS macro letters · 2024Article
- Cell response to extracellular matrix viscous energy dissipation outweighs high-rigidity sensing.Science advances · 2024Article
- Harnessing quantum light for microscopic biomechanical imaging of cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex.Ultrasonics sonochemistry · 2024Article
- Evaluating ECM stiffness and liver cancer radiation response via shear-wave elasticity in 3D culture models.Radiation oncology (London, England) · 2024Article
- Pharmacological Blockade of the Adenosine ACells · 2024Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Regulation of cell functions by the physical properties of the extracellular matrix (ECM) has emerged as a crucial contributor to development and disease. Two specific physical properties of the ECM, stiffness and dimensionality, each influence cell signaling and function. As these ECM physical properties are linked to other properties that also regulate cell behavior, e.g., integrin ligand density, parsing the specific contributions of ECM stiffness and dimensionality has proven difficult. Here we detail a simple protocol, which can be completed in 1-2 d, for combining three-dimensional (3D) ECM engagement with controlled underlying ECM stiffness. In these 'sandwich gels', cells are sandwiched between a 3D fibrillar ECM and an ECM-coupled polyacrylamide gel of defined compliance, allowing the study of the specific effects of ECM compliance on cell function in physiologically relevant 3D ECMs. This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.