ArticleJournal of proteome research2012
High-throughput isolation and characterization of untagged membrane protein complexes: outer membrane complexes of Desulfovibrio vulgaris.
Article in Journal of proteome research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
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- Reconstitution of a biofilm adhesin system from a sulfate-reducing bacterium inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reconstitution of a Biofilm Adhesin System from a Sulfate-Reducing Bacterium inbioRxiv : the preprint server for biology · 2023Article
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- Genomic insight into iron acquisition by sulfate-reducing bacteria in microaerophilic environments.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Characterization of a soluble library of themicroLife · 2023Article
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- Experimental evolution reveals nitrate tolerance mechanisms in Desulfovibrio vulgaris.The ISME journal · 2020Article
- Next-generation Interactomics: Considerations for the Use of Co-elution to Measure Protein Interaction Networks.Molecular & cellular proteomics : MCP · 2020Review
- Article
- Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.Journal of bacteriology · 2017Article
- Unintended Laboratory-Driven Evolution Reveals Genetic Requirements for Biofilm Formation bymBio · 2017Article
- Quantitative Tagless Copurification: A Method to Validate and Identify Protein-Protein Interactions.Molecular & cellular proteomics : MCP · 2016Article
- Bacterial Interactomes: Interacting Protein Partners Share Similar Function and Are Validated in Independent Assays More Frequently Than Previously Reported.Molecular & cellular proteomics : MCP · 2016Article
- Genetic basis for nitrate resistance in Desulfovibrio strains.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 1 country.
Funding
Abstract
Cell membranes represent the "front line" of cellular defense and the interface between a cell and its environment. To determine the range of proteins and protein complexes that are present in the cell membranes of a target organism, we have utilized a "tagless" process for the system-wide isolation and identification of native membrane protein complexes. As an initial subject for study, we have chosen the Gram-negative sulfate-reducing bacterium Desulfovibrio vulgaris. With this tagless methodology, we have identified about two-thirds of the outer membrane- associated proteins anticipated. Approximately three-fourths of these appear to form homomeric complexes. Statistical and machine-learning methods used to analyze data compiled over multiple experiments revealed networks of additional protein-protein interactions providing insight into heteromeric contacts made between proteins across this region of the cell. Taken together, these results establish a D. vulgaris outer membrane protein data set that will be essential for the detection and characterization of environment-driven changes in the outer membrane proteome and in the modeling of stress response pathways. The workflow utilized here should be effective for the global characterization of membrane protein complexes in a wide range of organisms.
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Registered trials
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