ArticleBiomedical engineering online2012
Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential.
Article in Biomedical engineering online, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Fibroblast-Derived Decellularized Extracellular Matrix as a Bioactive Substrate for Osteoblast Activation.ACS omega · 2026Article
- Biomimetic mineralization of electrospun elastin-like recombinamer nanofibers.Scientific reports · 2026Article
- Decoding cellular communication networks and signaling pathways in bone, skeletal muscle, and bone-muscle crosstalk through spatial transcriptomics in a young male mouse.Bone research · 2026Article
- Article
- Tension-Dominant Orthodontic Loading and Buccal Periodontal Phenotype Preservation: An Integrative Mechanobiological Model Supported by FEM and a Proof-of-Concept CBCT.Journal of functional biomaterials · 2026Article
- Investigating the Role of Exercise in Improving Bone Health Among Elderly Women with Osteopenia.Journal of functional morphology and kinesiology · 2025Article
- The Effect of Bone Mechanical Stress Caused by Electrical Stimulation-Induced Muscle Contraction on Osteocalcin Secretion.Biology · 2024Article
- Mechanically strained osteocyte-derived exosomes contained miR-3110-5p and miR-3058-3p and promoted osteoblastic differentiation.Biomedical engineering online · 2024Article
- Cancer cell response to extrinsic and intrinsic mechanical cue: opportunities for tumor apoptosis strategies.Regenerative biomaterials · 2024Review
- "Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.Bioactive materials · 2023Article
- Conditioned medium of PC‑3 prostate cancer cells affects microRNA and mRNA profiles in mechanically strained osteoblasts.Experimental and therapeutic medicine · 2023Article
- Biomaterials via peptide assembly: Design, characterization, and application in tissue engineering.Acta biomaterialia · 2022Review
- Enhancement of Bone Regeneration Through the Converse Piezoelectric Effect, A Novel Approach for Applying Mechanical Stimulation.Bioelectricity · 2021Review
- Diffuse microdamage in bone activates anabolic response by osteoblasts via involvement of voltage-gated calcium channels.Journal of bone and mineral metabolism · 2020Article
- Uniaxial Static Strain Promotes Osteoblast Proliferation and Bone Matrix Formation in Distraction Osteogenesis In Vitro.BioMed research international · 2020Article
- Vibration acceleration promotes endochondral formation during fracture healing through cellular chondrogenic differentiation.PloS one · 2020Article
- Combating osteoporosis and obesity with exercise: leveraging cell mechanosensitivity.Nature reviews. Endocrinology · 2019Review
- Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart.The Journal of clinical investigation · 2018Article
- Cyclic stretch enhances bone morphogenetic protein-2-induced osteoblastic differentiation through the inhibition of Hey1.International journal of molecular medicine · 2015Article
- Mineral trioxide aggregate induces osteoblastogenesis via Atf6.Bone reports · 2015Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity.
methodsMouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM.
resultsThe cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells.
conclusionMechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.
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