ArticleChemistry Central journal2012
Design of optimal solvent for extraction of bio-active ingredients from six varieties of Medicago sativa.
Article in Chemistry Central journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed.
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- Assessment of the antidiabetic potential of extract and novel phytoniosomes formulation of Tradescantia pallida leaves in the alloxan-induced diabetic mouse model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
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- Impact of Sprouting under Potassium Nitrate Priming on Nitrogen Assimilation and Bioactivity of ThreePlants (Basel, Switzerland) · 2021Article
- Contents and yields of copper, iron, manganese and zinc would be affected by lucerne age and cut.PeerJ · 2021Article
- Antioxidant Activity and Cytotoxicity ofBioResearch open access · 2020Article
- Antinociception of petroleum ether fraction derived from crude methanol extract of Melastoma malabathricum leaves and its possible mechanisms of action in animal models.BMC complementary and alternative medicine · 2016Article
- Phytochemical Compounds and Antioxidant Capacity of Tucum-Do-Cerrado (Bactris setosa Mart), Brazil's Native Fruit.Nutrients · 2016Article
- Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method.Molecules (Basel, Switzerland) · 2015Article
- Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves.BMC research notes · 2013Article
- Reverse phase chromatographic behaviour of major components in Capsicum Annuum extract.Chemistry Central journal · 2012Article
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5 authors.
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Abstract
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backgroundExtensive research has been performed worldwide and important evidences were collected to show the immense potential of plants used in various traditional therapeutic systems. The aim of this work is to investigate the different extracting solvents in terms of the influence of their polarity on the extracting ability of bioactive molecules (phenolic compounds) from the M. sativa flowers.
resultsThe total phenolic content of samples was determined using the Folin Ciocalteu (FC) procedure and their antioxidant activity was assayed through in vitro radical decomposing activity using the radical DPPH° assay (IUPAC name for DPPH is (phenyl)-(2,4,6-trinitrophenyl) iminoazanium). The results showed that water was better than methanol and acetic acid for extracting bioactive compounds, in particular for total phenolic compounds from the flowers of alfalfa. The average content of bioactive molecules in methanol extract was 263.5±1.02 mg GAE/100g of dry weight lyophilized extract. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. However, all extracts were free-radical inhibitors, but the water extract was more potent than the acetic and the methanol ones. The order of inhibitor effectiveness (expressed by IC50) proved to be: water extract (0.924mg/mL) > acetic acid extract (0.154mg/mL) > methanol (0.079mg/mL). The profiles of each extract (fingerprint) were characterized by FT-MIR spectroscopy.
conclusionsThe present study compares the fingerprint of different extracts of the M. sativa flowers, collected from the wild flora of Romania. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. The dependence of the extract composition on the solvent polarity (acetic acid vs. methanol vs. water) was revealed by UV-VIS spectrometry and Infrared fingerprint.
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