ArticleMolecular biology of the cell2012
Histone H1 compacts DNA under force and during chromatin assembly.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Insights from single-molecule force spectroscopy into chromatin topology.Biophysical reviews · 2026Review
- Protamine folds DNA into flowers and loop stacks.Biophysical journal · 2023Article
- Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast.mBio · 2023Article
- Monitoring the compaction of single DNA molecules inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- HIV-1 Preintegration Complex Preferentially Integrates the Viral DNA into Nucleosomes Containing Trimethylated Histone 3-Lysine 36 Modification and Flanking Linker DNA.Journal of virology · 2022Article
- Making Mitotic Chromosomes in a Test Tube.Epigenomes · 2022Review
- Chromatin sequesters pioneer transcription factor Sox2 from exerting force on DNA.Nature communications · 2022Article
- Single-stranded nucleic acid binding and coacervation by linker histone H1.Nature structural & molecular biology · 2022Article
- The Cell Adhesion Molecule L1 Interacts with Methyl CpG Binding Protein 2 via Its Intracellular Domain.International journal of molecular sciences · 2022Article
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- Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly.Nature communications · 2021Article
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- Centromere mechanical maturation during mammalian cell mitosis.Nature communications · 2019Article
- Epigenetics and autism spectrum disorder: A report of an autism case with mutation in H1 linker histone HIST1H1E and literature review.American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics · 2018Review
- The nucleus is irreversibly shaped by motion of cell boundaries in cancer and non-cancer cells.Journal of cellular physiology · 2018Article
- The nesprin-cytoskeleton interface probed directly on single nuclei is a mechanically rich system.Nucleus (Austin, Tex.) · 2017Article
- Correlation among DNA Linker Length, Linker Histone Concentration, and Histone Tails in Chromatin.Biophysical journal · 2016Article
- Linker histone H1 and H3K56 acetylation are antagonistic regulators of nucleosome dynamics.Nature communications · 2015Article
- Forced unraveling of chromatin fibers with nonuniform linker DNA lengths.Journal of physics. Condensed matter : an Institute of Physics journal · 2015Article
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Histone H1 binds to linker DNA between nucleosomes, but the dynamics and biological ramifications of this interaction remain poorly understood. We performed single-molecule experiments using magnetic tweezers to determine the effects of H1 on naked DNA in buffer or during chromatin assembly in Xenopus egg extracts. In buffer, nanomolar concentrations of H1 induce bending and looping of naked DNA at stretching forces below 0.6 pN, effects that can be reversed with 2.7-pN force or in 200 mM monovalent salt concentrations. Consecutive tens-of-nanometer bending events suggest that H1 binds to naked DNA in buffer at high stoichiometries. In egg extracts, single DNA molecules assemble into nucleosomes and undergo rapid compaction. Histone H1 at endogenous physiological concentrations increases the DNA compaction rate during chromatin assembly under 2-pN force and decreases it during disassembly under 5-pN force. In egg cytoplasm, histone H1 protects sperm nuclei undergoing genome-wide decondensation and chromatin assembly from becoming abnormally stretched or fragmented due to astral microtubule pulling forces. These results reveal functional ramifications of H1 binding to DNA at the single-molecule level and suggest an important physiological role for H1 in compacting DNA under force and during chromatin assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.