ArticleMolecular biology of the cell2012
Integrin α5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Structure and function of mouse lens suture examined by 2-photon fluorescence microscopic imaging.Scientific reports · 2026Article
- Zebrafish col4a1 loss-of-function models mirror key neurovascular and ocular features of COL4A1/A2 syndrome and enable human variants assessment in vivo.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- A genetic modifier links integrin α5 to the phenotypic variation in fibronectin 1a mutant zebrafish.PLoS genetics · 2025Article
- Live imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development.Cells & development · 2024Article
- Build me up optic cup: Intrinsic and extrinsic mechanisms of vertebrate eye morphogenesis.Developmental biology · 2021Review
- Single cell transcriptomics of the developing zebrafish lens and identification of putative controllers of lens development.Experimental eye research · 2021Article
- Identification of fibronectin 1 as a candidate genetic modifier in a Col4a1 mutant mouse model of Gould syndrome.Disease models & mechanisms · 2021Article
- PRPS polymerization influences lens fiber organization in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Optic cup morphogenesis requires neural crest-mediated basement membrane assembly.Development (Cambridge, England) · 2020Article
- LongAxis: A MATLAB-based program for 3D quantitative analysis of epithelial cell shape and orientation.Developmental biology · 2020Article
- Focal adhesions are essential to drive zebrafish heart valve morphogenesis.The Journal of cell biology · 2019Article
- Proteome-transcriptome analysis and proteome remodeling in mouse lens epithelium and fibers.Experimental eye research · 2019Article
- Aqp0a Regulates Suture Stability in the Zebrafish Lens.Investigative ophthalmology & visual science · 2018Article
- Feedback between tissue packing and neurogenesis in the zebrafish neural tube.Development (Cambridge, England) · 2018Article
- Loss of αB-crystallin function in zebrafish reveals critical roles in the development of the lens and stress resistance of the heart.The Journal of biological chemistry · 2018Article
- Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αA-crystallin mutants.PloS one · 2018Article
- β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis.Investigative ophthalmology & visual science · 2017Article
- The lens actin filament cytoskeleton: Diverse structures for complex functions.Experimental eye research · 2017Review
- Expression of Cataract-linked γ-Crystallin Variants in Zebrafish Reveals a Proteostasis Network That Senses Protein Stability.The Journal of biological chemistry · 2016Article
- Genomics of Rapid Incipient Speciation in Sympatric Threespine Stickleback.PLoS genetics · 2016Article
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Authors and funding
6 authors.
Funding
Abstract
Lens fiber formation and morphogenesis requires a precise orchestration of cell- extracellular matrix (ECM) and cell-cell adhesive changes in order for a lens epithelial cell to adopt a lens fiber fate, morphology, and migratory ability. The cell-ECM interactions that mediate these processes are largely unknown, and here we demonstrate that fibronectin1 (Fn1), an ECM component, and integrin α5, its cellular binding partner, are required in the zebrafish lens for fiber morphogenesis. Mutations compromising either of these proteins lead to cataracts, characterized by defects in fiber adhesion, elongation, and packing. Loss of integrin α5/Fn1 does not affect the fate or viability of lens epithelial cells, nor does it affect the expression of differentiation markers expressed in lens fibers, although nucleus degradation is compromised. Analysis of the intracellular mediators of integrin α5/Fn1 activity focal adhesion kinase (FAK) and integrin-linked kinase (ILK) reveals that FAK, but not ILK, is also required for lens fiber morphogenesis. These results support a model in which lens fiber cells use integrin α5 to migrate along a Fn-containing substrate on the apical side of the lens epithelium and on the posterior lens capsule, likely activating an intracellular signaling cascade mediated by FAK in order to orchestrate the cytoskeletal changes in lens fibers that facilitate elongation, migration, and compaction.
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Registered trials
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