ArticleMethods in molecular biology (Clifton, N.J.)2013
Three-dimensional culture of human breast epithelial cells: the how and the why.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 175 citations in OpenAlex.
- Construction and application of mammary and breast cancer organoids.Cell insight · 2026Review
- Microbe-associated molecular patterns differentially mediate carcinogenic alterations of the breast tissue in the context of obesity.Neoplasia (New York, N.Y.) · 2026Article
- Impact of lactation stage, maternal age, and parity on mammary epithelial cell populations in fresh milk.Scientific reports · 2025Article
- Review
- Mechanisms of breast cancer metastasis: the role of extracellular matrix.Molecular and cellular biochemistry · 2025Review
- Obesity increases DNA damage in the breast epithelium.Breast cancer research : BCR · 2025Article
- Organoid Cultures for the Study of Mammary Biology and Breast Cancer: The Promise and Challenges.Cold Spring Harbor perspectives in medicine · 2024Review
- Spheroid Model of Mammary Tumor Cells: Epithelial-Mesenchymal Transition and Doxorubicin Response.Biology · 2024Article
- Current status and challenges for cell-cultured milk technology: a systematic review.Journal of animal science and biotechnology · 2024Review
- Assessing the impact of extracellular matrix fiber orientation on breast cancer cellular metabolism.Cancer cell international · 2024Article
- The epithelial polarity axis controls the resting membrane potential and Cl- co-transport in breast glandular structures.Journal of cell science · 2024Article
- Follicular DNA Damage and Pesticide Exposure Among Latinx Children in Rural and Urban Communities.Exposure and health · 2024Article
- The microbiome: a link between obesity and breast cancer risk.Frontiers in microbiomes · 2024Review
- Next Generation CD44v6-Specific CAR-NK Cells Effective against Triple Negative Breast Cancer.International journal of molecular sciences · 2023Article
- Reversion of breast epithelial polarity alterations caused by obesity.NPJ breast cancer · 2023Article
- Influence of Simulated Microgravity on Mammary Epithelial Cells Grown as 2D and 3D Cultures.International journal of molecular sciences · 2023Article
- Multi-glycomic analysis of spheroid glycocalyx differentiates 2- and 3-dimensional cell models.Glycobiology · 2023Article
- Corneal epithelial basement membrane assembly is mediated by epithelial cells in coordination with corneal fibroblasts during wound healing.Molecular vision · 2023Article
- Over-expression of miR-183-5p or miR-492 triggers invasion and proliferation and loss of polarity in non-neoplastic breast epithelium.Scientific reports · 2022Article
- In situ 3D spatiotemporal measurement of soluble biomarkers in spheroid culture.In vitro models · 2022Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Organs are made of the organized assembly of different cell types that contribute to the architecture necessary for functional differentiation. In those with exocrine function, such as the breast, cell-cell and cell-extracellular matrix (ECM) interactions establish mechanistic constraints and a complex biochemical signaling network essential for differentiation and homeostasis of the glandular epithelium. Such knowledge has been elegantly acquired for the mammary gland by placing epithelial cells under three-dimensional (3D) culture conditions.Three-dimensional cell culture aims at recapitulating normal and pathological tissue architectures, hence providing physiologically relevant models to study normal development and disease. The specific architecture of the breast epithelium consists of glandular structures (acini) connected to a branched ductal system. A single layer of basoapically polarized luminal cells delineates ductal or acinar lumena at the apical pole. Luminal cells make contact with myoepithelial cells and, in certain areas at the basal pole, also with basement membrane (BM) components. In this chapter, we describe how this exquisite organization as well as stages of disorganization pertaining to cancer progression can be reproduced in 3D cultures. Advantages and limitations of different culture settings are discussed. Technical designs for induction of phenotypic modulations, biochemical analyses, and state-of-the-art imaging are presented. We also explain how signaling is regulated differently in 3D cultures compared to traditional two-dimensional (2D) cultures. We believe that using 3D cultures is an indispensable method to unravel the intricacies of human mammary functions and would best serve the fight against breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.