ArticleJournal of cell science2012
The functions of anionic phospholipids during clathrin-mediated endocytosis site initiation and vesicle formation.
Article in Journal of cell science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator.Molecular biology of the cell · 2026Article
- The conserved protein adaptors CALM/AP180 and FCHo1/2 cooperatively recruit Eps15 to promote the initiation of clathrin-mediated endocytosis in yeast.PLoS biology · 2024Article
- Different ER-plasma membrane tethers play opposing roles in autophagy of the cortical ER.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.bioRxiv : the preprint server for biology · 2023Article
- Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast.Cell structure and function · 2023Article
- Insights into Membrane Curvature Sensing and Membrane Remodeling by Intrinsically Disordered Proteins and Protein Regions.The Journal of membrane biology · 2022Review
- Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.Nature communications · 2021Article
- Cooperativity of membrane-protein and protein-protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis.Cellular and molecular life sciences : CMLS · 2021Article
- Spatial regulation of clathrin-mediated endocytosis through position-dependent site maturation.The Journal of cell biology · 2020Article
- Lipids inFrontiers in cellular and infection microbiology · 2020Review
- Phosphatidylserine is critical for vesicle fission during clathrin-mediated endocytosis.Journal of neurochemistry · 2020Article
- Molecular Mechanisms of Membrane Curvature Sensing by a Disordered Protein.Journal of the American Chemical Society · 2019Article
- Retrograde trafficking and quality control of yeast synaptobrevin, Snc1, are conferred by its transmembrane domain.Molecular biology of the cell · 2019Article
- Phosphatidate phosphatase regulates membrane phospholipid synthesis via phosphatidylserine synthase.Advances in biological regulation · 2018Review
- YeastThe Journal of biological chemistry · 2017Article
- Phospholipid flippases attenuate LPS-induced TLR4 signaling by mediating endocytic retrieval of Toll-like receptor 4.Cellular and molecular life sciences : CMLS · 2017Article
- Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast.G3 (Bethesda, Md.) · 2017Article
- Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2.The Journal of cell biology · 2017Article
- Clathrin-mediated endocytosis in budding yeast at a glance.Journal of cell science · 2016Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Live-cell imaging of endocytic protein dynamics in an mss4(ts) mutant, which has severely reduced PI(4,5)P2 levels, revealed that PI(4,5)P2 is required for endocytic membrane invagination but is less important for endocytic site initiation. We also demonstrated that, in various deletion mutants of genes encoding components of the Rcy1-Ypt31/32 GTPase pathway, endocytic proteins dynamically assemble not only on the plasma membrane but also on intracellular membrane compartments, which are likely derived from early endosomes. In rcy1Δ cells, fluorescent biosensors indicated that PI(4,5)P2 only localized to the plasma membrane while PS localized to both the plasma membrane and intracellular membranes. Furthermore, we found that polarized endocytic patch establishment is defective in the PS-deficient cho1Δ mutant. We propose that PS is important for directing endocytic proteins to the plasma membrane and that PI(4,5)P2 is required to facilitate endocytic membrane invagination.
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