ArticleThe Journal of investigative dermatology2013
Lysophosphatidic acid promotes cell migration through STIM1- and Orai1-mediated Ca2+(i) mobilization and NFAT2 activation.
Article in The Journal of investigative dermatology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- MICU2 up-regulation enhances tumor aggressiveness and metabolic reprogramming during colorectal cancer development.PLoS biology · 2024Article
- Regulation of CD8 T-cell signaling, metabolism, and cytotoxic activity by extracellular lysophosphatidic acid.Immunological reviews · 2023Review
- Similarities and Differences between the Orai1 Variants: Orai1α and Orai1β.International journal of molecular sciences · 2022Review
- Lysophosphatidic Acid Promotes the Expansion of Cancer Stem Cells via TRPC3 Channels in Triple-Negative Breast Cancer.International journal of molecular sciences · 2022Article
- Transient Receptor Potential Ankyrin 1 (TRPA1)-An Inflammation-Induced Factor in Human HaCaT Keratinocytes.International journal of molecular sciences · 2021Article
- LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.Nature communications · 2021Article
- Different agonists induce distinct single-channel conductance states in TRPV1 channels.The Journal of general physiology · 2018Article
- Rapid and dynamic arginylation of the leading edge β-actin is required for cell migration.Traffic (Copenhagen, Denmark) · 2018Article
- The role of Orai-STIM calcium channels in melanocytes and melanoma.The Journal of physiology · 2016Review
- STIM1 overexpression promotes colorectal cancer progression, cell motility and COX-2 expression.Oncogene · 2015Article
- Type II cyclic guanosine monophosphate-dependent protein kinase inhibits Rac1 activation in gastric cancer cells.Oncology letters · 2015Article
- Role of LPA and the Hippo pathway on apoptosis in salivary gland epithelial cells.Experimental & molecular medicine · 2014Article
- T-plastin expression downstream to the calcineurin/NFAT pathway is involved in keratinocyte migration.PloS one · 2014Article
- ORAI1 calcium channel orchestrates skin homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Regulation of CRAC channels by protein interactions and post-translational modification.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Lysophosphatidic acid (LPA) enhances cell migration and promotes wound healing in vivo, but the intracellular signaling pathways regulating these processes remain incompletely understood. Here we investigated the involvement of agonist-induced Ca(2+) entry and STIM1 and Orai1 proteins in regulating nuclear factor of activated T cell (NFAT) signaling and LPA-induced keratinocyte cell motility. As monitored by Fluo-4 imaging, stimulation with 10 μM LPA in 60 μM Ca(2+)(o) evoked Ca(2+)(i) transients owing to store release, whereas addition of LPA in physiological 1.2 mM Ca(2+)(o) triggered store release coupled to extracellular Ca(2+) entry. Store-operated Ca(2+) entry (SOCE) was blocked by the SOCE inhibitor diethylstilbestrol (DES), STIM1 silencing using RNA interference (RNAi), and expression of dominant/negative Orai1(R91W). LPA induced significant NFAT activation as monitored by nuclear translocation of green fluorescent protein-tagged NFAT2 and a luciferase reporter assay, which was impaired by DES, expression of Orai1(R91W), and inhibition of calcineurin using cyclosporin A (CsA). By using chemotactic migration assays, LPA-induced cell motility was significantly impaired by STIM1, CsA, and NFAT2 knockdown using RNAi. These data indicate that in conditions relevant to epidermal wound healing, LPA induces SOCE and NFAT activation through Orai1 channels and promotes cell migration through a calcineurin/NFAT2-dependent pathway.
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