ArticlePLoS computational biology2012
Determining host metabolic limitations on viral replication via integrated modeling and experimental perturbation.
Article in PLoS computational biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Acclimation temperature influences phage susceptibility in a toxin-producing strain ofMicrobiology spectrum · 2026Article
- Dynamic metabolic modelling of ATP allocation during viral infection.Journal of the Royal Society, Interface · 2026Article
- Metabolic remodeling of microorganisms by mobile genetic elements alters mutualistic community composition.mSystems · 2025Article
- Virocell resource manipulation under nutrient limitation.mSystems · 2025Article
- Viral plasticity facilitates host diversity in challenging environments.Nature communications · 2024Article
- Article
- Computational multigene interactions in virus growth and infection spread.Virus evolution · 2024Article
- Replication cycle timing determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system.PLoS pathogens · 2023Article
- Csx28 is a membrane pore that enhances CRISPR-Cas13b-dependent antiphage defense.Science (New York, N.Y.) · 2023Article
- Single-Cell Approach Reveals Intercellular Heterogeneity in Phage-Producing Capacities.Microbiology spectrum · 2023Article
- Time to lysis determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system.bioRxiv : the preprint server for biology · 2023Article
- Phenotypic flux: The role of physiology in explaining the conundrum of bacterial persistence amid phage attack.Virus evolution · 2022Review
- Article
- Inhibiting the reproduction of SARS-CoV-2 through perturbations in human lung cell metabolic network.Life science alliance · 2021Article
- Evolutionarily Stable Coevolution Between a Plastic Lytic Virus and Its Microbial Host.Frontiers in microbiology · 2021Article
- A Family of Viral Satellites Manipulates Invading Virus Gene Expression and Can Affect Cholera Toxin Mobilization.mSystems · 2020Article
- Hitchhiking, collapse, and contingency in phage infections of migrating bacterial populations.The ISME journal · 2020Article
- Evolutionary advantage of anti-parallel strand orientation of duplex DNA.Scientific reports · 2020Article
- Phage-specific metabolic reprogramming of virocells.The ISME journal · 2020Article
- Nitrogen sourcing during viral infection of marine cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Viral replication relies on host metabolic machinery and precursors to produce large numbers of progeny - often very rapidly. A fundamental example is the infection of Escherichia coli by bacteriophage T7. The resource draw imposed by viral replication represents a significant and complex perturbation to the extensive and interconnected network of host metabolic pathways. To better understand this system, we have integrated a set of structured ordinary differential equations quantifying T7 replication and an E. coli flux balance analysis metabolic model. Further, we present here an integrated simulation algorithm enforcing mutual constraint by the models across the entire duration of phage replication. This method enables quantitative dynamic prediction of virion production given only specification of host nutritional environment, and predictions compare favorably to experimental measurements of phage replication in multiple environments. The level of detail of our computational predictions facilitates exploration of the dynamic changes in host metabolic fluxes that result from viral resource consumption, as well as analysis of the limiting processes dictating maximum viral progeny production. For example, although it is commonly assumed that viral infection dynamics are predominantly limited by the amount of protein synthesis machinery in the host, our results suggest that in many cases metabolic limitation is at least as strict. Taken together, these results emphasize the importance of considering viral infections in the context of host metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.