ArticlePLoS computational biology2012
Membrane-elasticity model of Coatless vesicle budding induced by ESCRT complexes.
Article in PLoS computational biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Membrane free-energy landscapes derived from atomistic dynamics explain nonuniversal cholesterol-induced stiffening.PNAS nexus · 2023Article
- Dynamics of upstream ESCRT organization at the HIV-1 budding site.Biophysical journal · 2023Article
- Membrane free-energy landscapes derived from atomistic dynamics explain nonuniversal cholesterol-induced stiffening.bioRxiv : the preprint server for biology · 2023Article
- Protein crowding mediates membrane remodeling in upstream ESCRT-induced formation of intraluminal vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In Vitro Membrane Remodeling by ESCRT is Regulated by Negative Feedback from Membrane Tension.iScience · 2019Article
- Modeling Membrane Curvature Generation due to Membrane⁻Protein Interactions.Biomolecules · 2018Review
- Domes and cones: Adhesion-induced fission of membranes by ESCRT proteins.PLoS computational biology · 2018Article
- Biophysics of membrane curvature remodeling at molecular and mesoscopic lengthscales.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Negative charge and membrane-tethered viral 3B cooperate to recruit viral RNA dependent RNA polymerase 3DScientific reports · 2017Article
- Scaffolding the cup-shaped double membrane in autophagy.PLoS computational biology · 2017Article
- Design principles for robust vesiculation in clathrin-mediated endocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- CURVATURE-DRIVEN MOLECULAR FLOW ON MEMBRANE SURFACE.SIAM journal on applied mathematics · 2017Article
- Reverse-topology membrane scission by the ESCRT proteins.Nature reviews. Molecular cell biology · 2017Review
- Article
- A novel minimal in vitro system for analyzing HIV-1 Gag-mediated budding.Journal of biological physics · 2015Article
- Bud-neck scaffolding as a possible driving force in ESCRT-induced membrane budding.Biophysical journal · 2015Article
- Membrane manipulations by the ESCRT machinery.F1000Research · 2015Review
- Vfa1 binds to the N-terminal microtubule-interacting and trafficking (MIT) domain of Vps4 and stimulates its ATPase activity.The Journal of biological chemistry · 2014Article
- Ubiquitin-dependent sorting in endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Molecular mechanisms of the membrane sculpting ESCRT pathway.Cold Spring Harbor perspectives in biology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The formation of vesicles is essential for many biological processes, in particular for the trafficking of membrane proteins within cells. The Endosomal Sorting Complex Required for Transport (ESCRT) directs membrane budding away from the cytosol. Unlike other vesicle formation pathways, the ESCRT-mediated budding occurs without a protein coat. Here, we propose a minimal model of ESCRT-induced vesicle budding. Our model is based on recent experimental observations from direct fluorescence microscopy imaging that show ESCRT proteins colocalized only in the neck region of membrane buds. The model, cast in the framework of membrane elasticity theory, reproduces the experimentally observed vesicle morphologies with physically meaningful parameters. In this parameter range, the minimum energy configurations of the membrane are coatless buds with ESCRTs localized in the bud neck, consistent with experiment. The minimum energy configurations agree with those seen in the fluorescence images, with respect to both bud shapes and ESCRT protein localization. On the basis of our model, we identify distinct mechanistic pathways for the ESCRT-mediated budding process. The bud size is determined by membrane material parameters, explaining the narrow yet different bud size distributions in vitro and in vivo. Our membrane elasticity model thus sheds light on the energetics and possible mechanisms of ESCRT-induced membrane budding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.