ArticleProceedings of the National Academy of Sciences of the United States of America2012
Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 59 citations in OpenAlex.
- Generation of germline-transmitting transgenic sheep by piggyBac-mediated transgenesis using pronuclear and cytoplasmic gene injection approaches.Transgenic research · 2026Article
- Highly efficient and low-mosaicismiScience · 2026Article
- Inducible trophoblast-specific knockdown of mechanistic target of rapamycin impairs placental folate transport in mice.The Journal of nutritional biochemistry · 2026Article
- Article
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- In vivo placental gene modulation via sonoporation.Placenta · 2025Article
- Trophoblast-specific Deptor knockdown enhances trophoblast nutrient transport and fetal growth in mice.Acta physiologica (Oxford, England) · 2025Article
- Methodological landscape in the field of integration site identification of retroviruses and retroviral vectors.Frontiers in bioengineering and biotechnology · 2025Review
- Immunotherapy against colorectal cancer via delivery of anti-PD-L1 nanobody mRNA.eGastroenterology · 2025Article
- A microfluidic sucrose gap platform using trilaminar flow with on-chip switching and novel calibration: Challenges and limitations.Biomicrofluidics · 2025Article
- Polyethyleneimine-mediated gene transfection in porcine fetal fibroblasts.Animal reproduction · 2024Article
- Optimization of piggyBac transposon-mediated gene transfer method in common marmoset embryos.PloS one · 2023Article
- Ion Channel Drugs Suppress Cancer Phenotype in NG108-15 and U87 Cells: Toward Novel Electroceuticals for Glioblastoma.Cancers · 2022Article
- Establishment of an integrated automated embryonic manipulation system for producing genetically modified mice.Scientific reports · 2021Article
- Fine gene expression regulation by minor sequence variations downstream of the polyadenylation signal.Molecular biology reports · 2021Article
- Adeno-Associated Virus-Based Gene Therapy for Lifelong Correction of Genetic Disease.Human gene therapy · 2020Review
- RNA-guidedSynthetic biology (Oxford, England) · 2019Article
- Effects of AANAT overexpression on the inflammatory responses and autophagy activity in the cellular and transgenic animal levels.Autophagy · 2018Article
- Transposase-mediated gene modulation in the placenta.Placenta · 2017Review
- Pyrophosphate Supplementation Prevents Chronic and Acute Calcification in ABCC6-Deficient Mice.The American journal of pathology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
We have developed a unique method for mouse transgenesis. The transposase-enhanced pronuclear microinjection (PNI) technique described herein uses the hyperactive piggyBac transposase to insert a large transgene into the mouse genome. This procedure increased transgene integration efficiency by fivefold compared with conventional PNI or intracytoplasmic sperm injection-mediated transgenesis. Our data indicate that the transposase-enhanced PNI technique additionally requires fewer embryos to be microinjected than traditional methods to obtain transgenic animals. This transposase-mediated approach is also very efficient for single-cell embryo cytoplasmic injections, offering an easy-to-implement transgenesis method to the scientific community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.