ArticleNucleic acids research2013
RNA polymerase III mutants in TFIIFα-like C37 that cause terminator readthrough with no decrease in transcription output.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 37 citations in OpenAlex.
- Terminator-dependent facilitated recycling of RNA polymerase III provides gene-specific transcriptional activation in vivo.Science advances · 2026Article
- AbioRxiv : the preprint server for biology · 2024Article
- DNA-dependent RNA polymerases in plants.The Plant cell · 2023Article
- Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases.Nature reviews. Molecular cell biology · 2022Review
- An integrated model for termination of RNA polymerase III transcription.Science advances · 2022Article
- Evolution of the RNA Cleavage Subunit C11/RPC10, and Recycling by RNA Polymerase III.Journal of cellular immunology · 2022Article
- The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.Nucleic acids research · 2021Article
- Mechanism of RNA polymerase III termination-associated reinitiation-recycling conferred by the essential function of the N terminal-and-linker domain of the C11 subunit.Nature communications · 2021Article
- Structure of human RNA polymerase III elongation complex.Cell research · 2021Article
- Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.The EMBO journal · 2019Article
- RNA polymerase III subunits C37/53 modulate rU:dA hybrid 3' end dynamics during transcription termination.Nucleic acids research · 2019Article
- The Structures of Eukaryotic Transcription Pre-initiation Complexes and Their Functional Implications.Sub-cellular biochemistry · 2019Review
- Article
- Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes.Open biology · 2017Review
- Specialization versus conservation: How Pol I and Pol III use the conserved architecture of the pre-initiation complex for specialized transcription.Transcription · 2016Review
- Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo.PLoS genetics · 2016Article
- Transcribing RNA polymerase III observed by electron cryomicroscopy.The FEBS journal · 2016Review
- Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions.RNA (New York, N.Y.) · 2016Article
- A high density of cis-information terminates RNA Polymerase III on a 2-rail track.RNA biology · 2016Article
- Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
How eukaryotic RNA polymerases switch from elongation to termination is unknown. Pol III subunits Rpc53 and Rpc37 (C53/37) form a heterodimer homologous to TFIIFβ/α. C53/37 promotes efficient termination and together with C11 also mediates pol III recycling in vitro. We previously developed Schizosaccharomyces pombe strains that report on two pol III termination activities: RNA oligo(U) 3'-end cleavage, and terminator readthrough. We randomly mutagenized C53 and C37 and isolated many C37 mutants with terminator readthrough but no comparable C53 mutants. The majority of C37 mutants have strong phenotypes with up to 40% readthrough and map to a C-terminal tract previously localized near Rpc2p in the pol III active center while a minority represent a distinct class with weaker phenotype, less readthrough and 3'-oligo(U) lengthening. Nascent pre-tRNAs released from a terminator by C37 mutants have shorter 3'-oligo(U) tracts than in cleavage-deficient C11 double mutants indicating RNA 3'-end cleavage during termination. We asked whether termination deficiency affects transcription output in the mutants in vivo both by monitoring intron-containing nascent transcript levels and (14)C-uridine incorporation. Surprisingly, multiple termination mutants have no decrease in transcript output relative to controls. These data are discussed in context of current models of pol III transcription.
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