ArticleNucleic acids research2013
Rationally designed bidirectional promoter improves the evolutionary stability of synthetic genetic circuits.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 112 citations in OpenAlex.
- RUBY-BD: a highly efficient visual reporter system for assessing bidirectional promoter activity.Planta · 2026Article
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuits.Science advances · 2025Article
- Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria.Nature communications · 2025Article
- Genetic Entanglement Enables Ultrastable Biocontainment in the Mammalian Gut.ACS synthetic biology · 2025Article
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- From Natural Microbe Screening to Sustained Chitinase Activity in Exogenous Hosts.ACS synthetic biology · 2024Article
- Article
- Genetically stable kill-switch using "demon and angel" expression construct of essential genes.Frontiers in bioengineering and biotechnology · 2024Article
- Prolonging genetic circuit stability through adaptive evolution of overlapping genes.Nucleic acids research · 2023Article
- Integrase-mediated differentiation circuits improve evolutionary stability of burdensome and toxic functions in E. coli.Nature communications · 2022Article
- A genetic toolkit for co-expression of multiple proteins of diverse physiological implication.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Engineering gene overlaps to sustain genetic constructs in vivo.PLoS computational biology · 2021Article
- Design patterns for engineering genetic stability.Current opinion in biomedical engineering · 2021Article
- Bidirectional promoters: an enigmatic genome architecture and their roles in cancers.Molecular biology reports · 2021Review
- Modular Assembly of Ordered Hydrophilic Proteins Improve Salinity Tolerance inInternational journal of molecular sciences · 2021Article
- Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites.Nucleic acids research · 2021Article
- Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview.Foods (Basel, Switzerland) · 2020Review
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
- Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One problem with synthetic genes in genetically engineered organisms is that these foreign DNAs will eventually lose their functions over evolutionary time in absence of selective pressures. This general limitation can restrain the long-term study and industrial application of synthetic genetic circuits. Previous studies have shown that because of their crucial regulatory functions, prokaryotic promoters in synthetic genetic circuits are especially vulnerable to mutations. In this study, we rationally designed robust bidirectional promoters (BDPs), which are self-protected through the complementarity of their overlapping forward and backward promoter sequences on DNA duplex. When the transcription of a target non-essential gene (e.g. green fluorescent protein) was coupled to the transcription of an essential gene (e.g. antibiotic resistance gene) through the BDP, the evolutionary half-time of the gene of interest increases 4-10 times, depending on the strain and experimental conditions used. This design of using BDPs to increase the mutational stability of genetic circuits can be potentially applied to synthetic biology applications in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.