ArticleNucleic acids research2013
Quadruplex formation as a molecular switch to turn on intrinsically fluorescent nucleotide analogs.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Defined folding pattern of poly(rG) supports inherent ability to encode biological information.Biopolymers · 2024Article
- MutSβ-MutLβ-FANCJ axis mediates the restart of DNA replication after fork stalling at cotranscriptional G4/R-loops.Science advances · 2024Article
- Trinity of G-tetrads and origin of translation.Biology direct · 2022Article
- A Fluorescent G-Quadruplex Sensor for Chemical RNA Copying.Angewandte Chemie (International ed. in English) · 2018Article
- Isothermal amplification of long DNA segments by quadruplex priming amplification.Analytical methods : advancing methods and applications · 2018Article
- Monomolecular tetrahelix of polyguanine with a strictly defined folding pattern.Scientific reports · 2018Article
- Probing the competition between duplex and G-quadruplex/i-motif structures using a conformation-sensitive fluorescent nucleoside probe.Organic & biomolecular chemistry · 2018Article
- Fluorescence-based tools to probe G-quadruplexes in cell-free and cellular environments.RSC advances · 2018Article
- A practical guide to studying G-quadruplex structures using single-molecule FRET.Molecular genetics and genomics : MGG · 2017Review
- Alternative DNA structure formation in the mutagenic human c-MYC promoter.Nucleic acids research · 2017Article
- In What Ways Do Synthetic Nucleotides and Natural Base Lesions Alter the Structural Stability of G-Quadruplex Nucleic Acids?Journal of nucleic acids · 2017Review
- G-quadruplex recognition and remodeling by the FANCJ helicase.Nucleic acids research · 2016Article
- Stable Domain Assembly of a Monomolecular DNA Quadruplex: Implications for DNA-Based Nanoswitches.Biophysical journal · 2016Article
- Conformation-sensitive nucleoside analogues as topology-specific fluorescence turn-on probes for DNA and RNA G-quadruplexes.Nucleic acids research · 2015Article
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5 authors.
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Abstract
Quadruplexes are involved in the regulation of gene expression and are part of telomeres at the ends of chromosomes. In addition, they are useful in therapeutic and biotechnological applications, including nucleic acid diagnostics. In the presence of K(+) ions, two 15-mer sequences d(GGTTGGTGTGGTTGG) (thrombin binding aptamer) and d(GGGTGGGTGGGTGGG) (G3T) fold into antiparallel and parallel quadruplexes, respectively. In the present study, we measured the fluorescence intensity of one or more 2-aminopurine or 6-methylisoxanthopterin base analogs incorporated at loop-positions of quadruplex forming sequences to develop a detection method for DNA sequences in solution. Before quadruplex formation, the fluorescence is efficiently quenched in all cases. Remarkably, G3T quadruplex formation results in emission of fluorescence equal to that of a free base in all three positions. In the case of thrombin binding aptamer, the emission intensity depends on the location of the fluorescent nucleotides. Circular dichroism studies demonstrate that the modifications do not change the overall secondary structure, whereas thermal unfolding experiments revealed that fluorescent analogs significantly destabilize the quadruplexes. Overall, these studies suggest that quadruplexes containing fluorescent nucleotide analogs are useful tools in the development of novel DNA detection methodologies.
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