ArticleMolecular pharmacology2013
Distinct sensitivity of slo1 channel proteins to ethanol.
Article in Molecular pharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Planar Lipid Bilayers and Liposomes: Essential Models for Advancing Protein Structure and Function Research.Advances in experimental medicine and biology · 2026Review
- Changes in the Properties of Ethanol-Sensitive Molecular Targets During Maturation and Aging.Advances in experimental medicine and biology · 2025Review
- Article
- BK β1 subunit-dependent facilitation of ethanol inhibition of BK current and cerebral artery constriction is mediated by the β1 transmembrane domain 2.British journal of pharmacology · 2017Article
- The Slo(w) path to identifying the mitochondrial channels responsible for ischemic protection.The Biochemical journal · 2017Review
- Understanding the conformational motions of RCK gating rings.The Journal of general physiology · 2017Review
- Alcohol modulation of BK channel gating depends on β subunit composition.The Journal of general physiology · 2016Article
- Modulation of BK Channels by Ethanol.International review of neurobiology · 2016Review
- Ethanol Effect on BK Channels is Modulated by Magnesium.Alcoholism, clinical and experimental research · 2015Article
- Conserved single residue in the BK potassium channel required for activation by alcohol and intoxication in C. elegans.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- An alcohol-sensing site in the calcium- and voltage-gated, large conductance potassium (BK) channel.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Ethanol modulation of mammalian BK channels in excitable tissues: molecular targets and their possible contribution to alcohol-induced altered behavior.Frontiers in physiology · 2014Review
- Bisphenol A activates BK channels through effects on α and β1 subunits.Channels (Austin, Tex.) · 2014Article
- Distinct molecular targets including SLO-1 and gap junctions are engaged across a continuum of ethanol concentrations in Caenorhabditis elegans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Transduction of voltage and Ca2+ signals by Slo1 BK channels.Physiology (Bethesda, Md.) · 2013Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Ethanol levels reached in circulation during moderate-to-heavy alcohol intoxication (50-100 mM) modify Ca(2+)- and voltage-gated K(+) (BK) channel steady-state activity, eventually altering both physiology and behavior. Ethanol action on BK steady-state activity solely requires the channel-forming subunit slo1 within a bare lipid environment. To identify the protein regions that confer ethanol sensitivity to slo1, we tested the ethanol sensitivity of heterologously expressed slo1 and structurally related channels. Ethanol (50 mM) increased the steady-state activities of mslo1 and Ca(2+)-gated MthK, the latter after channel reconstitution into phospholipid bilayers. In contrast, 50-100 mM ethanol failed to alter the steady-state activities of Na(+)/Cl(-)-gated rslo2, H(+)-gated mslo3, and an mslo1/3 chimera engineered by joining the mslo1 region encompassing the N terminus to S6 with the mslo3 cytosolic tail domain (CTD). Collectively, data indicate that the slo family canonical design, which combines a transmembrane 6 (TM6) voltage-gated K(+) channel (K(V)) core with CTDs that empower the channel with ion-sensing, does not necessarily render ethanol sensitivity. In addition, the region encompassing the N terminus to the S0-S1 cytosolic loop (missing in MthK) is not necessary for ethanol action. Moreover, incorporation of both this region and an ion-sensing CTD to TM6 K(V) cores (a design common to mslo1, mslo3, and the mslo1/mslo3 chimera) is not sufficient for ethanol sensitivity. Rather, a CTD containing Ca(2+)-sensing regulator of conductance for K(+) domains seems to be critical to bestow K(V) structures, whether of TM2 (MthK) or TM6 (slo1), with sensitivity to intoxicating ethanol levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.