ArticleNature communications2012
Imaging the post-fusion release and capture of a vesicle membrane protein.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Small intracellular vesicles outperform small extracellular vesicles in uptake, drug delivery and retinal neuroprotection.Nature biomedical engineering · 2026Article
- Forty years of the adrenal chromaffin cell through ISCCB meetings around the world.Pflugers Archiv : European journal of physiology · 2023Review
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- The structure and spontaneous curvature of clathrin lattices at the plasma membrane.Developmental cell · 2021Article
- Structurally distinct endocytic pathways for B cell receptors in B lymphocytes.Molecular biology of the cell · 2020Article
- Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells.Lipids in health and disease · 2020Article
- Spatiotemporal organization and protein dynamics involved in regulated exocytosis of MMP-9 in breast cancer cells.The Journal of general physiology · 2019Article
- A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.The Journal of general physiology · 2019Review
- Local protein dynamics during microvesicle exocytosis in neuroendocrine cells.Molecular biology of the cell · 2018Article
- Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.Frontiers in cellular neuroscience · 2018Article
- Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.Nature communications · 2017Article
- Endocytic proteins are partitioned at the edge of the clathrin lattice in mammalian cells.Nature cell biology · 2017Article
- Superresolution Imaging of Clinical Formalin Fixed Paraffin Embedded Breast Cancer with Single Molecule Localization Microscopy.Scientific reports · 2017Article
- Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins.Nature communications · 2015Article
- Super-resolution microscopy of the synaptic active zone.Frontiers in cellular neuroscience · 2015Article
- Article
- Advances in imaging ultrastructure yield new insights into presynaptic biology.Frontiers in cellular neuroscience · 2015Review
- Flat clathrin lattices: stable features of the plasma membrane.Molecular biology of the cell · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The molecular mechanism responsible for capturing, sorting and retrieving vesicle membrane proteins following triggered exocytosis is not understood. Here we image the post-fusion release and then capture of a vesicle membrane protein, the vesicular acetylcholine transporter, from single vesicles in living neuroendocrine cells. We combine these measurements with super-resolution interferometric photo-activation localization microscopy and electron microscopy, and modelling to map the nanometer-scale topography and architecture of the structures responsible for the transporter's capture following exocytosis. We show that after exocytosis, the transporter rapidly diffuses into the plasma membrane, but most travels only a short distance before it is locally captured over a dense network of membrane-resident clathrin-coated structures. We propose that the extreme density of these structures acts as a short-range diffusion trap. They quickly sequester diffusing vesicle material and limit its spread across the membrane. This system could provide a means for clathrin-mediated endocytosis to quickly recycle vesicle proteins in highly excitable cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.