ArticleInvestigative ophthalmology & visual science2012
From blood islands to blood vessels: morphologic observations and expression of key molecules during hyaloid vascular system development.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 34 citations in OpenAlex.
- Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens.Experimental eye research · 2024Article
- Single-Cell Characterization of the Frizzled 5 (Fz5) Mutant Mouse and Human Persistent Fetal Vasculature (PFV).Investigative ophthalmology & visual science · 2023Article
- Resident immune cells of the avascular lens: Mediators of the injury and fibrotic response of the lens.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Selective Requirements for Vascular Endothelial Cells and Circulating Factors in the Regulation of Retinal Neurogenesis.Frontiers in cell and developmental biology · 2021Article
- An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Microglia in the developing retina.Neural development · 2019Review
- Longitudinal OCT and OCTA monitoring reveals accelerated regression of hyaloid vessels in retinal degeneration 10 (rd10) mice.Scientific reports · 2019Article
- Functional OCT angiography reveals early physiological dysfunction of hyaloid vasculature in developing mouse eye.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Building Blood Vessels with Vascular Progenitor Cells.Trends in molecular medicine · 2018Review
- A Modular Strategy to Engineer Complex Tissues and Organs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Ocular Histopathology and Immunohistochemical Analysis in the Oldest Known Individual with Autosomal Dominant Vitreoretinochoroidopathy.Ophthalmology. Retina · 2018Article
- Development of the hyaloid, choroidal and retinal vasculatures in the fetal human eye.Progress in retinal and eye research · 2018Review
- Article
- Stem cell-derived vasculature: A potent and multidimensional technology for basic research, disease modeling, and tissue engineering.Biochemical and biophysical research communications · 2016Review
- In vivo analysis of hyaloid vasculature morphogenesis in zebrafish: A role for the lens in maturation and maintenance of the hyaloid.Developmental biology · 2014Article
- Interaction between pericytes and endothelial cells leads to formation of tight junction in hyaloid vessels.Molecules and cells · 2013Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe mode of development of the human hyaloid vascular system (HVS) remains unclear. Early studies suggested that these blood vessels formed by vasculogenesis, while the current concept seems to favor angiogenesis as the mode of development. We examined embryonic and fetal human HVS using a variety of techniques to gain new insights into formation of this vasculature.
methodsEmbryonic and fetal human eyes from 5.5 to 12 weeks gestation (WG) were prepared for immunohistochemical analysis or for light and electron microscopy. Immunolabeling of sections with a panel of antibodies directed at growth factors, transcription factors, and hematopoietic stem cell markers was employed.
resultsLight microscopic examination revealed free blood islands (BI) in the embryonic vitreous cavity (5.5-7 WG). Giemsa stain revealed that BI were aggregates of mesenchymal cells and primitive nucleated erythroblasts. Free cells were also observed. Immunolabeling demonstrated that BI were composed of mesenchymal cells that expressed hemangioblast markers (CD31, CD34, C-kit, CXCR4, Runx1, and VEGFR2), erythroblasts that expressed embryonic hemoglobin (Hb-ε), and cells that expressed both. Few cells were proliferating as determined by lack of Ki67 antigen. As development progressed (12 WG), blood vessels became more mature structurally with pericyte investment and basement membrane formation. Concomitantly, Hb-ε and CXCR4 expression was down-regulated and von Willebrand factor expression was increased with the formation of Weibel-Palade bodies.
conclusionsOur results support the view that the human HVS, like the choriocapillaris, develops by hemo-vasculogenesis, the process by which vasculogenesis, erythropoiesis, and hematopoiesis occur simultaneously from common precursors, hemangioblasts.
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