ArticleMicrobial cell factories2012
Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 44 citations in OpenAlex.
- High efficient production of adenine in Bacillus subtilis with novel metabolic engineering strategies.World journal of microbiology & biotechnology · 2026Article
- Nucleotide Metabolism in Health and Disease.MedComm · 2026Review
- Glycine Drives IMP Production in Hybrid Bream (Megalobrama Amblycephala ♀ × Culter Alburnus ♂) Via Concerted Action: Activating glycyl-tRNA Synthetase and Fueling the Pentose Phosphate Pathway.Marine biotechnology (New York, N.Y.) · 2026Article
- Non-volatile and volatile compound analyses revealed the effect of oregano essential oil on the flavor characteristics of beef.Frontiers in nutrition · 2026Article
- Development of a CRISPR/Cas9 genome editing toolbox forSynthetic and systems biotechnology · 2025Article
- Metabolomics Analysis of the Spoilage Characteristics ofFoods (Basel, Switzerland) · 2025Article
- The difference between young and older ducks: Amino acid, free fatty acid, nucleotide compositions and breast muscle proteome.Food chemistry: X · 2025Article
- Exploration of the link between gut microbiota and purinergic signalling.Purinergic signalling · 2023Review
- Application of multi-omic features clustering and pathway enrichment to clarify the impact of vitamin B2 supplementation on broiler caeca microbiome.Frontiers in microbiology · 2023Article
- Insights into the Metabolic Response ofInternational journal of molecular sciences · 2022Article
- De novo purine nucleotide biosynthesis mediated by MoAde4 is required for conidiation, host colonization and pathogenicity in Magnaporthe oryzae.Applied microbiology and biotechnology · 2022Article
- Biomolecules, Fatty Acids, Meat Quality, and Growth Performance of Slow-Growing Chickens in an Organic Raising System.Animals : an open access journal from MDPI · 2022Article
- The impact of PrsA over-expression on theFrontiers in microbiology · 2022Article
- Cysteine Proteome Reveals Response to Endogenous Oxidative Stress inInternational journal of molecular sciences · 2021Article
- Ribosome Profiling and RNA Sequencing Reveal Genome-Wide Cellular Translation and Transcription Regulation Under Osmotic Stress inFrontiers in microbiology · 2021Article
- Overexpression of Phosphoribosyl Pyrophosphate Synthase Enhances Resistance ofFrontiers in plant science · 2021Article
- Article
- The Microenvironment Is a Critical Regulator of Muscle Stem Cell Activation and Proliferation.Frontiers in cell and developmental biology · 2019Review
- A new genome-scale metabolic model ofBiotechnology for biofuels · 2017Article
- Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines.Metabolic engineering · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPurine nucleotides exhibit various functions in cellular metabolism. Besides serving as building blocks for nucleic acid synthesis, they participate in signaling pathways and energy metabolism. Further, IMP and GMP represent industrially relevant biotechnological products used as flavor enhancing additives in food industry. Therefore, this work aimed towards the accumulation of IMP applying targeted genetic engineering of Corynebacterium glutamicum.
resultsBlocking of the degrading reactions towards AMP and GMP lead to a 45-fold increased intracellular IMP pool of 22 μmol g(CDW)⁻¹. Deletion of the pgi gene encoding glucose 6-phosphate isomerase in combination with the deactivated AMP and GMP generating reactions, however, resulted in significantly decreased IMP pools (13 μmol g(CDW)⁻¹). Targeted metabolite profiling of the purine biosynthetic pathway further revealed a metabolite shift towards the formation of the corresponding nucleobase hypoxanthine (102 μmol g(CDW)⁻¹) derived from IMP degradation.
conclusionsThe purine biosynthetic pathway is strongly interconnected with various parts of the central metabolism and therefore tightly controlled. However, deleting degrading reactions from IMP to AMP and GMP significantly increased intracellular IMP levels. Due to the complexity of this pathway further degradation from IMP to the corresponding nucleobase drastically increased suggesting additional targets for future strain optimization.
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