ArticleJournal of the American Chemical Society2012
Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cells.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Optically switchable CLEAR probes enable rapid, biocompatible and high-efficiency fluorophore exchange for ultra-plex, high-resolution immunofluorescence imaging.Chemical science · 2026Article
- Review
- Bidirectional linkage of DNA barcodes for the multiplexed mapping of higher-order protein interactions in cells.Nature biomedical engineering · 2024Article
- Site-directed conjugation of single-stranded DNA to affinity proteins: quantifying the importance of conjugation strategy.Chemical science · 2024Article
- Massively parallel single-cell sequencing of diverse microbial populations.Nature methods · 2024Article
- Recent methodological advances towards single-cell proteomics.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2023Review
- PhotocleavableChemical reviews · 2023Review
- Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers.Nature communications · 2023Article
- Modification of Cysteine-Substituted Antibodies Using Enzymatic Oxidative Coupling Reactions.Bioconjugate chemistry · 2023Article
- Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes.Nature biotechnology · 2022Article
- Multiplexed Single-Cell in Situ Protein Profiling.ACS measurement science au · 2022Review
- Multiplexed single-cell analysis of FNA allows accurate diagnosis of salivary gland tumors.Cancer cytopathology · 2022Review
- Cyclic microchip assay for measurement of hundreds of functional proteins in single neurons.Nature communications · 2022Article
- PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements.Nature communications · 2022Article
- DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling.Nano letters · 2021Article
- Single-cell proteomic analysis.WIREs mechanisms of disease · 2021Review
- Advances in Imaging Modalities, Artificial Intelligence, and Single Cell Biomarker Analysis, and Their Applications in Cytopathology.Frontiers in medicine · 2021Review
- Deterministic droplet codingLab on a chip · 2020Article
- A Cleavable CJournal of the American Chemical Society · 2020Article
- New technology on the horizon: Fast analytical screening technique FNA (FAST-FNA) enables rapid, multiplex biomarker analysis in head and neck cancers.Cancer cytopathology · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout. As a proof of principle, we demonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.
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Registered trials
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