ArticleOpen biology2012
Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.
Article in Open biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- Biomolecules Generated During Programmed Cell Death (PCD) Enhance the Capacity of Proliferating Ustilago maydis Cells to Overcome the Negative Impacts of Non-PCD Necromass.Microbial ecology · 2026Article
- Initial nutrient condition determines the recovery speed of quiescent cells in fission yeast.Heliyon · 2024Article
- Perturbed fatty-acid metabolism is linked to localized chromatin hyperacetylation, increased stress-response gene expression and resistance to oxidative stress.PLoS genetics · 2023Article
- Is There a Histone Code for Cellular Quiescence?Frontiers in cell and developmental biology · 2021Review
- Metabolomics of human fasting: new insights about old questions.Open biology · 2020Article
- A Geometric Clustering Tool (AGCT) to robustly unravel the inner cluster structures of time-series gene expressions.PloS one · 2020Article
- Co-Operation between Aneuploidy and Metabolic Changes in Driving Tumorigenesis.International journal of molecular sciences · 2019Review
- Diverse metabolic reactions activated during 58-hr fasting are revealed by non-targeted metabolomic analysis of human blood.Scientific reports · 2019Article
- Cell Lysate Microarray for Mapping the Network of Genetic Regulators for Histone Marks.Molecular & cellular proteomics : MCP · 2018Article
- CoA synthase regulates mitotic fidelity via CBP-mediated acetylation.Nature communications · 2018Article
- Simultaneous and absolute quantification of nucleoside triphosphates using liquid chromatography-triple quadrupole tandem mass spectrometry.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Article
- Understanding human thiol dioxygenase enzymes: structure to function, and biology to pathology.International journal of experimental pathology · 2017Review
- Functional and regulatory profiling of energy metabolism in fission yeast.Genome biology · 2016Article
- Nuclear pyruvate kinase M2 complex serves as a transcriptional coactivator of arylhydrocarbon receptor.Nucleic acids research · 2016Article
- Comparative Proteomic Analysis of Differential Proteins in Response to Aqueous Extract of Quercus infectoria Gall in Methicillin-Resistant Staphylococcus aureus.International journal of proteomics · 2016Article
- Modeling human Coenzyme A synthase mutation in yeast reveals altered mitochondrial function, lipid content and iron metabolism.Microbial cell (Graz, Austria) · 2015Article
- Regulation of a dynamic interaction between two microtubule-binding proteins, EB1 and TIP150, by the mitotic p300/CBP-associated factor (PCAF) orchestrates kinetochore microtubule plasticity and chromosome stability during mitosis.The Journal of biological chemistry · 2013Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Biosynthesis of coenzyme A (CoA) requires a five-step process using pantothenate and cysteine in the fission yeast Schizosaccharomyces pombe. CoA contains a thiol (SH) group, which reacts with carboxylic acid to form thioesters, giving rise to acyl-activated CoAs such as acetyl-CoA. Acetyl-CoA is essential for energy metabolism and protein acetylation, and, in higher eukaryotes, for the production of neurotransmitters. We isolated a novel S. pombe temperature-sensitive strain ppc1-537 mutated in the catalytic region of phosphopantothenoylcysteine synthetase (designated Ppc1), which is essential for CoA synthesis. The mutant becomes auxotrophic to pantothenate at permissive temperature, displaying greatly decreased levels of CoA, acetyl-CoA and histone acetylation. Moreover, ppc1-537 mutant cells failed to restore proliferation from quiescence. Ppc1 is thus the product of a super-housekeeping gene. The ppc1-537 mutant showed combined synthetic lethal defects with five of six histone deacetylase mutants, whereas sir2 deletion exceptionally rescued the ppc1-537 phenotype. In synchronous cultures, ppc1-537 cells can proceed to the S phase, but lose viability during mitosis failing in sister centromere/kinetochore segregation and nuclear division. Additionally, double-strand break repair is defective in the ppc1-537 mutant, producing fragile broken DNA, probably owing to diminished histone acetylation. The CoA-supported metabolism thus controls the state of chromosome DNA.
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