ArticlePloS one2012
Apocynin-treatment reverses hyperoxaluria induced changes in NADPH oxidase system expression in rat kidneys: a transcriptional study.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 40 citations in OpenAlex.
- Exploring cerebrospinal fluid metabolites, cognitive function, and brain atrophy: Insights from Mendelian randomization.Open medicine (Warsaw, Poland) · 2025Article
- Bioinformatics identifies key genes and potential therapeutic targets in the pathological mechanism of oxidative stress in Randall's plaque.Scientific reports · 2024Article
- The Altered Proteomic Landscape in Renal Tubular Epithelial Cells under High Oxalate Stimulation.Biology · 2024Article
- Risk factors of urolithiasis: A hospital-based retrospective study.Journal of family medicine and primary care · 2024Article
- Randall's plaque and calcium oxalate stone formation: role for immunity and inflammation.Nature reviews. Nephrology · 2021Review
- Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.Cell cycle (Georgetown, Tex.) · 2020Article
- Modulatory effect of 4-phenyl butyric acid on hyperoxaluria-induced renal injury and inflammation.Molecular and cellular biochemistry · 2019Article
- Total flavonoids of Desmodium styracifolium attenuates the formation of hydroxy-L-proline-induced calcium oxalate urolithiasis in rats.Urolithiasis · 2018Article
- Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress.Oxidative medicine and cellular longevity · 2018Article
- Kidney Stone Disease: An Update on Current Concepts.Advances in urology · 2018Review
- Article
- MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress.Oxidative medicine and cellular longevity · 2017Article
- M1/M2-macrophage phenotypes regulate renal calcium oxalate crystal development.Scientific reports · 2016Article
- Kidney stones.Nature reviews. Disease primers · 2016Review
- Involvement of renin-angiotensin-aldosterone system in calcium oxalate crystal induced activation of NADPH oxidase and renal cell injury.World journal of urology · 2016Article
- Antilithic effects of extracts from different polarity fractions of Desmodium styracifolium on experimentally induced urolithiasis in rats.Urolithiasis · 2015Article
- Osteogenic changes in kidneys of hyperoxaluric rats.Biochimica et biophysica acta · 2015Article
- Activation of the NLRP3 inflammasome in association with calcium oxalate crystal induced reactive oxygen species in kidneys.The Journal of urology · 2015Article
- Unified theory on the pathogenesis of Randall's plaques and plugs.Urolithiasis · 2015Article
- Osteopontin knockdown in the kidneys of hyperoxaluric rats leads to reduction in renal calcium oxalate crystal deposition.Urolithiasis · 2014Article
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5 authors at 2 institutions in 1 country.
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Abstract
purposeWe have previously shown that production of reactive oxygen species (ROS) is an important contributor to renal injury and inflammation following exposure to oxalate (Ox) or calcium-oxalate (CaOx) crystals. The present study was conducted, utilizing global transcriptome analyses, to determine the effect of Apocynin on changes in the NADPH oxidase system activated in kidneys of rats fed a diet leading to hyperoxaluria and CaOx crystal deposition. APPROACH: Age-, sex- and weight-matched rats were either fed regular rat chow or regular rat chow supplemented with 5% w/w hydroxy-L-proline (HLP). Half of the rats on the HLP diet were also placed on Apocynin-supplemented H(2)O. After 28 days, each rat was euthanized, their kidneys freshly explanted and dissected to obtain both cortex and medulla tissues. Total RNA was extracted from each tissue and subjected to genomic microarrays to obtain global transcriptome data. KEGG was used to identify gene clusters with differentially expressed genes. Immunohistochemistry was used to confirm protein expressions of selected genes.
resultsGenes encoding both membrane- and cytosolic-NADPH oxidase complex-associated proteins, together with p21rac and Rap1a, were coordinately up-regulated significantly in both renal medulla and cortex tissues in the HLP-fed rats compared to normal healthy untreated controls. Activation of NADPH oxidase appears to occur via the angiotensin-II/angiotensin-II receptor-2 pathway, although the DAG-PKC pathway of neutrophils may also contribute. Immuno histochemical staining confirmed up-regulated gene expressions. Simultaneously, genes encoding ROS scavenger proteins were down-regulated. HLP-fed rats receiving Apocynin had a complete reversal in the differential-expression of the NADPH oxidase system genes, despite showing similar levels of hyperoxaluria.
conclusionsA strong up-regulation of an oxidative/respiratory burst involving the NADPH oxidase system, activated via the angiotensin-II and most likely the DAG-PKC pathways, occurs in kidneys of hyperoxaluric rats. Apocynin treatment reversed this activation without affecting the levels of hyperoxaluria.
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