ArticlePloS one2012
Measurement and 3D-visualization of cell-cycle length using double labelling with two thymidine analogues applied in early heart development.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Human Cardiac Development.Advances in experimental medicine and biology · 2024Article
- Review
- The sodium channel NaActa physiologica (Oxford, England) · 2020Article
- The palette of techniques for cell cycle analysis.FEBS letters · 2020Review
- Development of the human heart.American journal of medical genetics. Part C, Seminars in medical genetics · 2020Review
- Application of three-dimensional reconstruction and printing as an elective course for undergraduate medical students: an exploratory trial.Surgical and radiologic anatomy : SRA · 2019Article
- Nkx2-5 Second Heart Field Target Gene Ccdc117 Regulates DNA Metabolism and Proliferation.Scientific reports · 2019Article
- Considerations for Measurement of Embryonic Organ Growth.Anatomical record (Hoboken, N.J. : 2007) · 2019Article
- Three-Dimensional Portable Document Format (3D PDF) in Clinical Communication and Biomedical Sciences: Systematic Review of Applications, Tools, and Protocols.JMIR medical informatics · 2018Review
- Proteomic analysis of cardiac ventricles: baso-apical differences.Molecular and cellular biochemistry · 2018Article
- Growth and Morphogenesis during Early Heart Development in Amniotes.Journal of cardiovascular development and disease · 2017Review
- Article
- Cardiac looping may be driven by compressive loads resulting from unequal growth of the heart and pericardial cavity. Observations on a physical simulation model.Frontiers in physiology · 2014Article
- The circadian clock in cancer development and therapy.Progress in molecular biology and translational science · 2013Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organ development is a complex spatial process in which local differences in cell proliferation rate play a key role. Understanding this role requires the measurement of the length of the cell cycle at every position of the three-dimensional (3D) structure. This measurement can be accomplished by exposing the developing embryo to two different thymidine analogues for two different durations immediately followed by tissue fixation. This paper presents a method and a dedicated computer program to measure the resulting labelling indices and subsequently calculate and visualize local cell cycle lengths within the 3D morphological context of a developing organ. By applying this method to the developing heart, we show a large difference in cell cycle lengths between the early heart tube and the adjacent mesenchyme of the pericardial wall. Later in development, a local increase in cell size was found to be associated with a decrease in cell cycle length in the region where the chamber myocardium starts to develop. The combined application of halogenated-thymidine double exposure and image processing enables the automated study of local cell cycle parameters in single specimens in a full 3D context. It can be applied in a wide range of research fields ranging from embryonic development to tissue regeneration and cancer research.
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