ArticleProceedings of the National Academy of Sciences of the United States of America2012
Force measurements on cargoes in living cells reveal collective dynamics of microtubule motors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed.
- Modelling nano-particle-PEG-NLS complexes for nucleus-targeted drug delivery: revisiting the multi-dynein nano-cargo transport on microtubules.Drug delivery · 2026Article
- Many dynein teams collectively generate high forces during the transport of large organelles.Biophysical journal · 2026Article
- Kinesin-Induced Buckling Reveals the Limits of Microtubule Self-Repair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Kinesin-1 autoinhibition tunes cargo transport by motor ensembles.Biophysical journal · 2025Article
- Measuring molecular forces inside living cells using magnetic tweezers.Biophysical reviews · 2025Review
- Deciphering the intracellular forces shaping mitochondrial motion.Scientific reports · 2024Article
- Optogenetic control of kinesin-1, -2, -3 and dynein reveals their specific roles in vesicular transport.Cell reports · 2024Article
- Kinesin-1-transported liposomes prefer to go straight in 3D microtubule intersections by a mechanism shared by other molecular motors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- Vesicles driven by dynein and kinesin exhibit directional reversals without regulators.Nature communications · 2023Article
- Single-molecule techniques to visualize and to characterize liquid-liquid phase separation and phase transition.Acta biochimica et biophysica Sinica · 2023Article
- On the use of thermal forces to probe kinesin's response to force.Frontiers in molecular biosciences · 2023Article
- Kinetics of phagosome maturation is coupled to their intracellular motility.Communications biology · 2022Article
- Article
- Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.iScience · 2022Article
- Establishing neuronal polarity: microtubule regulation during neurite initiation.Oxford open neuroscience · 2022Review
- Endocytic proteins with prion-like domains form viscoelastic condensates that enable membrane remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Nano-Particles Carried by Multiple Dynein Motors Self-Regulate Their Number of Actively Participating Motors.International journal of molecular sciences · 2021Article
- On and off controls within dynein-dynactin on native cargoes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport.Molecular biology of the cell · 2021Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Many cellular cargoes move bidirectionally along microtubules, driven by teams of plus- and minus-end-directed motor proteins. To probe the forces exerted on cargoes during intracellular transport, we examined latex beads phagocytosed into living mammalian macrophages. These latex bead compartments (LBCs) are encased in membrane and transported along the cytoskeleton by a complement of endogenous kinesin-1, kinesin-2, and dynein motors. The size and refractive index of LBCs makes them well-suited for manipulation with an optical trap. We developed methods that provide in situ calibration of the optical trap in the complex cellular environment, taking into account any variations among cargoes and local viscoelastic properties of the cytoplasm. We found that centrally and peripherally directed forces exerted on LBCs are of similar magnitude, with maximum forces of ~20 pN. During force events greater than 10 pN, we often observe 8-nm steps in both directions, indicating that the stepping of multiple motors is correlated. These observations suggest bidirectional transport of LBCs is driven by opposing teams of stably bound motors that operate near force balance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.