ArticleProceedings of the National Academy of Sciences of the United States of America2012
Genotype of a historic strain of Mycobacterium tuberculosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Multidisciplinary Exploration of Computed-Tomographic and Ancient-DNA Data of an Iron Age Skull From Latvia With Multiple Lytic Bone Lesions: Differential Diagnosis Between Metastatic Carcinoma, Multiple Myeloma and Skeletal Tuberculosis.Ecology and evolution · 2026Article
- Insight into blood proteinase-inhibitor system and pathogenesis of renal tuberculosis induced by phylogenomically different Mycobacterium tuberculosis strains in rabbit model.BMC nephrology · 2025Article
- Antimycobacterial Activity of Essential Oils from BulgarianPharmaceutics · 2024Article
- A multifaceted interplay between virulence, drug resistance, and the phylogeographic landscape ofMicrobiology spectrum · 2023Article
- The Evolution of Diagnostic Techniques in the Paleopathology of Tuberculosis: A Scoping Review.Pathogens & immunity · 2023Article
- Molecular genetic and geochemical assays reveal severe contamination of drinking water reservoirs at the ancient Maya city of Tikal.Scientific reports · 2020Article
- Genetic diversity and phylogenetic structure of four Tibeto-Burman-speaking populations in Tibetan-Yi corridor revealed by insertion/deletion polymorphisms.Molecular genetics & genomic medicine · 2020Article
- Screening methods for detection of ancient Mycobacterium tuberculosis complex fingerprints in next-generation sequencing data derived from skeletal samples.GigaScience · 2019Article
- Molecular archaeoparasitology identifies cultural changes in the Medieval Hanseatic trading centre of Lübeck.Proceedings. Biological sciences · 2018Article
- Ancient pathogen DNA in human teeth and petrous bones.Ecology and evolution · 2018Article
- Comprehensive analysis of microorganisms accompanying human archaeological remains.GigaScience · 2017Article
- Article
- Insights into human evolution from ancient and contemporary microbiome studies.Current opinion in genetics & development · 2016Review
- Archaeogenetics in evolutionary medicine.Journal of molecular medicine (Berlin, Germany) · 2016Review
- Paleomicrobiology: a Snapshot of Ancient Microbes and Approaches to Forensic Microbiology.Microbiology spectrum · 2016Review
- Molecular studies on ancient M. tuberculosis and M. leprae: methods of pathogen and host DNA analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- Ancient genomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- A new era in palaeomicrobiology: prospects for ancient dental calculus as a long-term record of the human oral microbiome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Screening ancient tuberculosis with qPCR: challenges and opportunities.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Article
- Complete genome sequence of Mycobacterium tuberculosis K from a Korean high school outbreak, belonging to the Beijing family.Standards in genomic sciences · 2015Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of ancient DNA in paleopathological studies of tuberculosis has largely been restricted to confirmation of disease identifications made by skeletal analysis; few attempts at obtaining genotype data from archaeological samples have been made because of the need to perform different PCRs for each genetic locus being studied in an ancient DNA extract. We used a next generation sequencing approach involving hybridization capture directed at specific polymorphic regions of the Mycobacterium tuberculosis genome to identify a detailed genotype for a historic strain of M. tuberculosis from an individual buried in the 19th century St. George's Crypt, Leeds, West Yorkshire, England. We obtained 664,500 sequencing by oligonucleotide ligation and detection (SOLiD) reads that mapped to the targeted regions of the M. tuberculosis genome; the coverage included 218 of 247 SNPs, 10 of 11 insertion/deletion regions, and the repeat elements IS1081 and IS6110. The accuracy of the SOLiD data was checked by conventional PCRs directed at 11 SNPs and two insertion/deletions. The data placed the historic strain of M. tuberculosis in a group that is uncommon today, but it is known to have been present in North America in the early 20th century. Our results show the use of hybridization capture followed by next generation sequencing as a means of obtaining detailed genotypes of ancient varieties of M. tuberculosis, potentially enabling meaningful comparisons between strains from different geographic locations and different periods in the past.
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