ArticleMolecular & cellular proteomics : MCP2013
Comparative proteomics of two life cycle stages of stable isotope-labeled Trypanosoma brucei reveals novel components of the parasite's host adaptation machinery.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed.
- Cross-species comparisons in a unified medium suggest broadly stable glycosome-linked enzyme levels under nutrient and oxygen variation.Frontiers in parasitology · 2026Article
- Hsp90 and associates shaping parasite biology.mSphere · 2025Review
- Discovery of effectors for casein kinase signaling in the African trypanosome.Scientific reports · 2025Article
- Immunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei reveals conserved and novel activities, including in the control of surface antigen expression needed for immune evasion by antigenic variation.Nucleic acids research · 2023Article
- Tackling Sleeping Sickness: Current and Promising Therapeutics and Treatment Strategies.International journal of molecular sciences · 2023Review
- Subcellular protein localisation ofWellcome open research · 2023Article
- Characterization of adenine phosphoribosyltransferase (APRT) activity in Trypanosoma brucei brucei: Only one of the two isoforms is kinetically active.PLoS neglected tropical diseases · 2022Article
- Article
- Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome.PloS one · 2021Article
- Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation iniScience · 2020Article
- The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Defeating the trypanosomatid trio: proteomics of the protozoan parasites causing neglected tropical diseases.RSC medicinal chemistry · 2020Review
- Small Molecule Inhibitors Targeting the Heat Shock Protein System of Human Obligate Protozoan Parasites.International journal of molecular sciences · 2019Review
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Proteome turnover in the bloodstream and procyclic forms ofWellcome open research · 2019Article
- The Hsp70/J-protein machinery of the African trypanosome, Trypanosoma brucei.Cell stress & chaperones · 2019Article
- Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.PLoS neglected tropical diseases · 2018Article
- Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans.Parasites & vectors · 2018Article
- TelAP1 links telomere complexes with developmental expression site silencing in African trypanosomes.Nucleic acids research · 2018Article
- UDP-glycosyltransferase genes in trypanosomatid genomes have diversified independently to meet the distinct developmental needs of parasite adaptations.BMC evolutionary biology · 2018Article
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6 authors.
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Abstract
Trypanosoma brucei developed a sophisticated life cycle to adapt to different host environments. Although developmental differentiation of T. brucei has been the topic of intensive research for decades, the mechanisms responsible for adaptation to different host environments are not well understood. We developed stable isotope labeling by amino acids in cell culture in trypanosomes to compare the proteomes of two different life cycle stages. Quantitative comparison of 4364 protein groups identified many proteins previously not known to be stage-specifically expressed. The identification of stage-specific proteins helps to understand how parasites adapt to different hosts and provides new insights into differences in metabolism, gene regulation, and cell architecture. A DEAD-box RNA helicase, which is highly up-regulated in the bloodstream form of this parasite and which is essential for viability and proper cell cycle progression in this stage is described as an example.
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