ArticleMolecular systems biology2012
Sequestration-based bistability enables tuning of the switching boundaries and design of a latch.
Article in Molecular systems biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 98 citations in OpenAlex.
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- A cross-species inducible system for enhanced protein expression and multiplexed metabolic pathway fine-tuning in bacteria.Nucleic acids research · 2025Article
- Sequence-Based Protein Design: A Review of Using Statistical Models to Characterize Coevolutionary Traits for Developing Hybrid Proteins as Genetic Sensors.International journal of molecular sciences · 2024Review
- A MoClo-Compatible Toolbox of ECF Sigma Factor-Based Regulatory Switches for Proteobacterial Chassis.Biodesign research · 2024Article
- Regulation strategies for two-output biomolecular networks.Journal of the Royal Society, Interface · 2023Article
- Origin and functional role of antisense transcription in endogenous and exogenous retroviruses.Retrovirology · 2023Review
- Rethinking Biosynthesis of Aclacinomycin A.Molecules (Basel, Switzerland) · 2023Review
- Frequency spectra and the color of cellular noise.Nature communications · 2022Article
- A balancing act in transcription regulation by response regulators: titration of transcription factor activity by decoy DNA binding sites.Nucleic acids research · 2021Article
- Programming ultrasensitive threshold response through chemomechanical instability.Nature communications · 2021Article
- Autonomous and Assisted Control for Synthetic Microbiology.International journal of molecular sciences · 2020Review
- Regulatory control of the Streptococcus mutans HdrRM LytTR Regulatory System functions via a membrane sequestration mechanism.Molecular microbiology · 2020Article
- Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch.Molecular systems biology · 2020Article
- Quantitative and Predictive Genetic Parts for Plant Synthetic Biology.Frontiers in plant science · 2020Review
- A chemically fueled non-enzymatic bistable network.Nature communications · 2019Article
- Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Extracytoplasmic Function σ Factors Can Be Implemented as Robust Heterologous Genetic Switches in Bacillus subtilis.iScience · 2019Article
- Engineering orthogonal synthetic timer circuits based on extracytoplasmic function σ factors.Nucleic acids research · 2018Article
- Antithetic proportional-integral feedback for reduced variance and improved control performance of stochastic reaction networks.Journal of the Royal Society, Interface · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural biological systems have evolved a diverse array of switches to realize their strategies for environmental response and development. Emerging applications of synthetic biology have begun to exploit such switches to achieve increasingly sophisticated designed behaviors. However, not all switch architectures allow facile design of the switching and memory properties. Furthermore, not all designs are built from components for which large families of variants exist, a requirement for building many orthogonal switch variants. Therefore, there is a critical need from genetic engineers for scalable strategies that yield custom bistable switches. Here, we use a sigma factor and its cognate anti-sigma factor to experimentally verify that ultrasensitivity from sequestration combined with positive feedback is sufficient to build a bistable switch. We show that sequestration allows us to predictably tune the switching boundaries, and we can easily tune our switch to function as a set-reset latch that can be toggled between two states by a pulse of inducer input.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.