ArticleStem cell research2013
Sodium-dependent vitamin C transporter SVCT2: expression and function in bone marrow stromal cells and in osteogenesis.
Article in Stem cell research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Ascorbic Acid Modulates Collagen Properties in Glucocorticoid-Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation.Advanced healthcare materials · 2026Article
- Cholesterol metabolism on stem cell fate determination: a frontier horizon.Stem cell research & therapy · 2025Review
- Role of vitamins in the development and treatment of osteoporosis (Review).International journal of molecular medicine · 2025Review
- Molecular Pharmacology of Vitamin C and Relevance to Health and Obesity-A Narrative Review.International journal of molecular sciences · 2024Review
- Intra-articular injection of ascorbic acid enhances microfracture-mediated cartilage repair.Scientific reports · 2024Article
- Low level of Vitamin C and dysregulation of Vitamin C transporter might be involved in the severity of COVID-19 Infection.Aging and disease · 2021Article
- Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer.Journal of Cancer · 2021Article
- Vitamin C supplementation for the treatment of osteoarthritis: perspectives on the past, present, and future.Therapeutic advances in chronic disease · 2021Review
- Gender-Specific Response in Pain and Function to Biologic Treatment of Knee Osteoarthritis: A Gender-Bias-Mitigated, Observational, Intention-to-Treat Study at Two Years.Stem cells international · 2021Article
- Picolinic acid, a tryptophan oxidation product, does not impact bone mineral density but increases marrow adiposity.Experimental gerontology · 2020Article
- The Emerging Role of Vitamin C as a Treatment for Sepsis.Nutrients · 2020Review
- MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- Roles for miRNAs in osteogenic differentiation of bone marrow mesenchymal stem cells.Stem cell research & therapy · 2019Review
- Role of MicroRNA-141 in the Aging Musculoskeletal System: A Current Overview.Mechanisms of ageing and development · 2019Review
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- Differentiation of Promonocytic U937 Cells to Monocytes Is Associated with Reduced Mitochondrial Transport of Ascorbic Acid.Oxidative medicine and cellular longevity · 2018Article
- Kynurenine, a Tryptophan Metabolite That Accumulates With Age, Induces Bone Loss.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
- Therapeutic potential of mesenchymal stem cell based therapy for osteoarthritis.Clinical and translational medicine · 2016Review
- Osteoporosis, vitamin C intake, and physical activity in Korean adults aged 50 years and over.Journal of physical therapy science · 2016Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
Ascorbic acid (Vitamin C) has a critical role in bone formation and osteoblast differentiation, but very little is known about the molecular mechanisms of ascorbic acid entry into bone marrow stromal cells (BMSCs). To address this gap in knowledge, we investigated the identity of the transport system that is responsible for the uptake of ascorbic acid into bone marrow stromal cells (BMSCs). First, we examined the expression of the two known isoforms of the sodium-coupled ascorbic acid transporter, namely SVCT1 and SVCT2, in BMSCs (Lin-ve Sca1+ve) and bone at the mRNA level. Only SVCT2 mRNA was detected in BMSCs and bone. Uptake of ascorbic acid in BMSCs was Na(+)-dependent and saturable. In order to define the role of SVCT2 in BMSC differentiation into osteoblasts, BMSCs were stimulated with osteogenic media for different time intervals, and the activity of SVCT2 was monitored by ascorbic acid uptake. SVCT2 expression was up-regulated during the osteogenic differentiation of BMSCs; the expression was maximal at the earliest phase of differentiation. Subsequently, osteogenesis was inhibited in BMSCs upon knock-down of SVCT2 by lentivirus shRNA. We also found that the expression of the SVCT2 could be negatively or positively modulated by the presence of oxidant (Sin-1) or antioxidant (Ascorbic acid) compounds, respectively, in BMSCs. Furthermore, we found that this transporter is also regulated with age in mouse bone. These data show that SVCT2 plays a vital role in the osteogenic differentiation of BMSCs and that its expression is altered under conditions associated with redox reaction. Our findings could be relevant to bone tissue engineering and bone related diseases such as osteoporosis in which oxidative stress and aging plays important role.
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Registered trials
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