ArticleArteriosclerosis, thrombosis, and vascular biology2012
Blood clots are rapidly assembled hemodynamic sensors: flow arrest triggers intraluminal thrombus contraction.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
74 citing papers in PubMed, 102 citations in OpenAlex.
- Mechanical Characterization of Fibrin-Based Blood Clot Analogues for Modeling Intracerebral Hemorrhage.Annals of biomedical engineering · 2026Article
- Transforming stroke care: the promise of feedback-guided thrombolytic nanomedicine.Journal of nanobiotechnology · 2026Review
- Rupture mechanics of blood clot fibrin fibers: A coarse-grained model study.Journal of the mechanics and physics of solids · 2025Article
- Article
- A novel technique to quantify the kinetics of blood clot contraction based on the expulsion of fluorescently labeled albumin into serum.Journal of thrombosis and haemostasis : JTH · 2024Article
- Hemostasis-on-a-chip / incorporating the endothelium in microfluidic models of bleeding.Platelets · 2023Review
- Probing interactions of red blood cells and contracting fibrin platelet clots.Biophysical journal · 2023Article
- Activation of Piezo1 channels in compressed red blood cells augments platelet-driven contraction of blood clots.Journal of thrombosis and haemostasis : JTH · 2023Article
- Platelet factor XIII-A regulates platelet function and promotes clot retraction and stability.Research and practice in thrombosis and haemostasis · 2023Article
- Image-based flow simulation of platelet aggregates under different shear rates.PLoS computational biology · 2023Article
- Investigating thrombin-loaded fibrin in whole blood clot microfluidic assay via fluorogenic peptide.Biophysical journal · 2023Article
- Recent Advances in Blood Cell-Inspired and Clot Targeted Thrombolytic Therapies.Journal of tissue engineering and regenerative medicine · 2023Review
- Blood clot contraction: Mechanisms, pathophysiology, and disease.Research and practice in thrombosis and haemostasis · 2023Article
- Effects of diabetes mellitus complicated by admission hyperglycemia on clot histological composition and ultrastructure in patients with acute ischemic stroke.BMC neurology · 2022Article
- Extent of intravital contraction of arterial and venous thrombi and pulmonary emboli.Blood advances · 2022Article
- Multifunctional exosomes derived from bone marrow stem cells for fulfilled osseointegration.Frontiers in chemistry · 2022Review
- A three-dimensional multiscale model for the prediction of thrombus growth under flow with single-platelet resolution.PLoS computational biology · 2022Article
- Point of care whole blood microfluidics for detecting and managing thrombotic and bleeding risks.Lab on a chip · 2021Review
- Thrombosis and Hemodynamics: external and intrathrombus gradients.Current opinion in biomedical engineering · 2021Article
- Clot Retraction: Cellular Mechanisms and Inhibitors, Measuring Methods, and Clinical Implications.Biomedicines · 2021Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
objectiveBlood clots form under flow during intravascular thrombosis or vessel leakage. Prevailing hemodynamics influence thrombus structure and may regulate contraction processes. A microfluidic device capable of flowing human blood over a side channel plugged with collagen (± tissue factor) was used to measure thrombus permeability (κ) and contraction at controlled transthrombus pressure drops. METHODS AND
resultsThe collagen (κ(collagen)=1.98 × 10(-11) cm(2)) supported formation of a 20-µm thick platelet layer, which unexpectedly underwent massive platelet retraction on flow arrest. This contraction resulted in a 5.34-fold increase in permeability because of collagen restructuring. Without stopping flow, platelet deposits (no fibrin) had a permeability of κ(platelet)=5.45 × 10(-14) cm(2) and platelet-fibrin thrombi had κ(thrombus)=2.71 × 10(-14) cm(2) for ΔP=20.7 to 23.4 mm Hg, the first ever measurements for clots formed under arterial flow (1130 s(-1) wall shear rate). Platelet sensing of flow cessation triggered a 4.6- to 6.5-fold (n=3, P<0.05) increase in contraction rate, which was also observed in a rigid, impermeable parallel-plate microfluidic device. This triggered contraction was blocked by the myosin IIA inhibitor blebbistatin and by inhibitors of thromboxane A2 (TXA(2)) and ADP signaling. In addition, flow arrest triggered platelet intracellular calcium mobilization, which was blocked by TXA(2)/ADP inhibitors. As clots become occlusive or blood pools following vessel leakage, the flow diminishes, consequently allowing full platelet retraction.
conclusionsFlow dilution of ADP and thromboxane regulates platelet contractility with prevailing hemodynamics, a newly defined flow-sensing mechanism to regulate clot function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.