ArticleMolecular biology of the cell2012
SNAP-23 regulates phagosome formation and maturation in macrophages.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Opposing roles for SNAP23 and SNAP25 in mediating MR1 trafficking and antigen presentation.bioRxiv : the preprint server for biology · 2026Article
- Identification of the role of SNARE proteins in rAAV vector production through interaction with the viral MAAP.Molecular therapy. Methods & clinical development · 2025Article
- Article
- VAMP5 promotes Fcγ receptor-mediated phagocytosis and regulates phagosome maturation in macrophages.Molecular biology of the cell · 2024Article
- SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.Communications biology · 2024Article
- Major histocompatibility complex class I assembly within endolysosomal pathways.Current opinion in immunology · 2023Review
- Transcriptome Changes and Potential Immunotoxicity Analysis in RAW264.7 Macrophages Caused by Bisphenol F.Frontiers in pharmacology · 2022Article
- Better Together: Current Insights Into Phagosome-Lysosome Fusion.Frontiers in immunology · 2021Review
- Examining the Underappreciated Role ofFrontiers in immunology · 2021Review
- Regulatory Mechanism of SNAP23 in Phagosome Formation and Maturation.Yonago acta medica · 2020Review
- Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYXAnnals of translational medicine · 2020Article
- Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages.Biology open · 2020Article
- Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages.Molecular biology of the cell · 2019Article
- Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor-mediated phagosome formation and maturation in macrophages.Molecular biology of the cell · 2018Article
- GhSNAP33, a t-SNARE Protein FromFrontiers in plant science · 2018Article
- Oxidized phagosomal NOX2 complex is replenished from lysosomes.Journal of cell science · 2017Article
- The endoplasmic reticulum and casein-containing vesicles contribute to milk fat globule membrane.Molecular biology of the cell · 2016Article
- The Membrane Phospholipid Binding Protein Annexin A2 Promotes Phagocytosis and Nonlytic Exocytosis of Cryptococcus neoformans and Impacts Survival in Fungal Infection.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Resistance to anticancer vaccination effect is controlled by a cancer cell-autonomous phenotype that disrupts immunogenic phagocytic removal.Oncotarget · 2015Article
- SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles.Biology open · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptosomal associated protein of 23 kDa (SNAP-23), a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), has been implicated in phagocytosis by macrophages. For elucidation of its precise role in this process, a macrophage line overexpressing monomeric Venus-tagged SNAP-23 was established. These cells showed enhanced Fc receptor-mediated phagocytosis. Detailed analyses of each process of phagocytosis revealed a marked increase in the production of reactive oxygen species within phagosomes. Also, enhanced accumulation of a lysotropic dye, as well as augmented quenching of a pH-sensitive fluorophore were observed. Analyses of isolated phagosomes indicated the critical role of SNAP-23 in the functional recruitment of the NADPH oxidase complex and vacuolar-type H(+)-ATPase to phagosomes. The data from the overexpression experiments were confirmed by SNAP-23 knockdown, which demonstrated a significant delay in phagosome maturation and a reduction in uptake activity. Finally, for analyzing whether phagosomal SNAP-23 entails a structural change in the protein, an intramolecular Förster resonance energy transfer (FRET) probe was constructed, in which the distance within a TagGFP2-TagRFP was altered upon close approximation of the N-termini of its two SNARE motifs. FRET efficiency on phagosomes was markedly enhanced only when VAMP7, a lysosomal SNARE, was coexpressed. Taken together, our results strongly suggest the involvement of SNAP-23 in both phagosome formation and maturation in macrophages, presumably by mediating SNARE-based membrane traffic.
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