ArticleThe Journal of biological chemistry2012
Intracellular endothelin type B receptor-driven Ca2+ signal elicits nitric oxide production in endothelial cells.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Role of CaCancer medicine · 2026Article
- Combined Effects of Diosmin, Hesperidin,International journal of molecular sciences · 2025Article
- Endothelin 3/EDNRB signaling induces thermogenic differentiation of white adipose tissue.Nature communications · 2024Article
- Dopamine internalization via UptakeFrontiers in pharmacology · 2024Article
- A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody-Drug Conjugate for Targeting Melanoma.Cancer research · 2023Article
- Minimal contribution of IPActa pharmacologica Sinica · 2020Article
- Hyaluronan Production by Renomedullary Interstitial Cells: Influence of Endothelin, Angiotensin II and Vasopressin.International journal of molecular sciences · 2017Article
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- Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition.Journal of cell science · 2015Article
- Differential activation of intracellular versus plasmalemmal CB2 cannabinoid receptors.Biochemistry · 2014Article
- Endothelin-1 activation of the endothelin B receptor modulates pulmonary endothelial CX3CL1 and contributes to pulmonary angiogenesis in experimental hepatopulmonary syndrome.The American journal of pathology · 2014Article
- Direct evidence of intracrine angiotensin II signaling in neurons.American journal of physiology. Cell physiology · 2014Article
- The basic biology of redoxosomes in cytokine-mediated signal transduction and implications for disease-specific therapies.Biochemistry · 2014Review
- Differential activation of cultured neonatal cardiomyocytes by plasmalemmal versus intracellular G protein-coupled receptor 55.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Endothelin-1 exerts its actions via activation of ET(A) and ET(B) G(q/11) protein-coupled receptors, located in the plasmalemma, cytoplasm, and nucleus. Although the autocrine/paracrine nature of endothelin-1 signaling has been extensively studied, its intracrine role has been largely attributed to interaction with receptors located on nuclear membranes and the nucleoplasm. Because ET(B) receptors have been shown to be targeted to endolysosomes, we used intracellular microinjection and concurrent imaging methods to test their involvement in Ca(2+) signaling and subsequential NO production. We provide evidence that microinjected endothelin-1 produces a dose-dependent elevation in cytosolic calcium concentration in ET(B)-transfected cells and endothelial cells; this response is sensitive to ET(B) but not ET(A) receptor blockade. In endothelial cells, the endothelin-1-induced Ca(2+) response is abolished upon endolysosomal but not Golgi disruption. Moreover, the effect is prevented by inhibition of microautophagy and is sensitive to inhibitors of the phospholipase C and inositol 1,4,5-trisphosphate receptor. Furthermore, intracellular endothelin-1 increases nitric oxide via an ET(B)-dependent mechanism. Our results indicate for the first time that intracellular endothelin-1 activates endolysosomal ET(B) receptors and increase cytosolic Ca(2+) and nitric oxide production. Endothelin-1 acts in an intracrine fashion on endolysosomal ET(B) to induce nitric oxide formation, thus modulating endothelial function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.